1. Group Name
Ecoli1
Group member name

2. Isolates Sample name Country Collection date ec11-4632_c1 France ec
c236-11
Germany ec ec
2009

3. Result of species identification (KmerFinder)
Strain
Species acc. to Grad et al 2012
Predicted species
Closest matching strain
Template coverage
ec _c1 ec
c236-11 ec ec

4. Representative KmerFinder result

5. Result of Multilocus Sequence Typing (MLST)
Strain
ST acc. to Grad et al 2012
Predicted ST
ec _c1 ec
c236-11 ec ec

6. Representative Multilocus Sequence Typing result

7. Result of serotyping ec11-4632_c1 ec11-5538 c236-11 ec11-3677
Strain
Serotype acc. to Grad et al 2012
Predicted serotype
ec _c1 ec
c236-11 ec ec

8. Representative serotyping result

9. Result of antibiotics resistance analysis
Isolate
Ampicillin (blaZ, blaTem-1B)
Third-generation cephalosporins (blaCTX-M-15)
Streptomycin (strA, strB, aadA)
Nalidixic acid (chromosomal mutations)
Tetracycline (tetC, tetM, tetR, tetS, tetA)
ec _c1 ec c ec ec
Isolate
Cotri-moxazole (dfr)
Carbape-nems (blaNDM)
Ciprofloxacin (chromosomal mutations)
Chloram-phenicol (cat)
Kanamycin (aph(3’)-II/aph(3’)-III)
Gentamicin (aac(6')-aph(2''))
ec _c1 ec c ec ec
How does it compare to the results presented in Grad et al 2012?

10. Virulence factors Isolate stx2 (stx2a variant) aggR pic stx1 eae hlyA
astA
papG
dsbD
ec _c1 ec c ec ec
Indicate with , e.g., a “X”, if the gene was present or not
Which pathovar does the outbreak isolates seem to belong to?

11. Result of plasmid analysis
Isolate
IncFII
IncFIB
IncI1
IncP
IncQ1
ec _c1 ec c ec ec
For each strain write which node the replicon was found on. If not found, leave the cell empty.
Which plasmid is the blaCTX-M-15 gene on?
Which plasmid are the aggR gene on?

12. Distance matrix made by NDtree and tree made with neighbour-joining

13. Distance matrix made by CSIPhylogeny and tree made with neighbour-joining