1. Volume 106, Issue 6, Pages 697-708 (September 2001)
Serial Regulation of Transcriptional Regulators in the Yeast Cell Cycle 
Itamar Simon, John Barnett, Nancy Hannett, Christopher T Harbison, Nicola J Rinaldi, Thomas L Volkert, John J Wyrick, Julia Zeitlinger, David K Gifford, Tommi S Jaakkola, Richard A Young 
Cell 
Volume 106, Issue 6, Pages (September 2001)
DOI: /S (01)

2. Figure 1 Cell Cycle Transcriptional Regulators: Study Design
(A) The stages of the cell cycle are depicted together with yeast cell morphology (brown) and transcriptional regulators (blue). The transcriptional regulators are positioned at the stage during which they have been reported to function (Breeden, 2000; Mendenhall and Hodge, 1998).
(B) Schematic summary of the experimental design. Detailed protocols can be obtained at the authors' website at and through the Cell website at
(C) A scatter plot of Cy5 versus Cy3 intensities for a control experiment in which aliquots of whole cell extract (WCE) were independently labeled with Cy3 and Cy5 and hybridized to a DNA microarray containing all yeast intergenic regions. The red and blue lines border the regions with confidence levels of p < and p < 0.01, respectively.
(D) A scatter plot of an experiment in which the Fkh2 IP-enriched DNA was labeled with Cy5 and the WCE was labeled with Cy3. The red and blue lines border the regions with confidence levels of p < and p < 0.01, respectively. The spots whose values have confidence levels of p < represent promoters most likely bound by the Fkh2 factor. Scatter plots for all the experiments can be found at the authors' web site
Cell  , DOI: ( /S (01) )

3. Figure 2
Genome-wide Location of the Nine Cell Cycle Transcription Factors
(A) 213 of the 800 cell cycle genes whose promoter regions were bound by a myc-tagged version of at least one of the nine cell cycle transcription factors (p < 0.001) are represented as horizontal lines. The weight-averaged binding ratios are displayed using a blue and white color scheme (genes with p value < are displayed in blue). The expression ratios of an α factor synchronization time course from Spellman et al. (1998) are displayed using a red (induced) and green (repressed) color scheme.
(B) The circle represents a smoothed distribution of the transcription timing (phase) of the 800 cell cycle genes (Spellman et al., 1998). The intensity of the red color, normalized by the maximum intensity value for each factor, represents the fraction of genes expressed at that point that are bound by a specific activator. The similarity in the distribution of color for specific factors (with Swi4, Swi6, and Mbp1, for example) shows that these factors bind to genes that are expressed during the same time frame
Cell  , DOI: ( /S (01) )

4. Figure 3
Transcriptional Regulation of Cell Cycle Transcription Factor Genes
(A) Summary of previous evidence for regulation of cell cycle transcription factor genes and CLN3 by transcriptional regulators (Althoefer et al., 1995; Foster et al., 1993; Koranda et al., 2000; Kumar et al., 2000; Kuo and Grayhack, 1994; Loy et al., 1999; Lydall et al., 1991; Mackay et al., 2001; McInerny et al., 1997; Pic et al., 2000; Zhu et al., 2000). The relationships between the transcription factors and their target genes are indicated by red arrows; solid lines represent evidence for direct regulation by these factors, and dashed lines represent inferences from indirect evidence. The blue arrows represent posttranscriptional regulation by Cln3/Cdc28 (Dirick et al., 1995).
(B) Model for the closed regulatory circuit produced by cell cycle transcriptional regulators based on genome-wide binding data. The genome-wide location data indicate that each group of transcriptional activators regulates activators acting in the next cell cycle stage. The red arrows represent binding of a transcription factor to the promoter of another regulatory factor. The blue arrows represent posttranscriptional regulation
Cell  , DOI: ( /S (01) )

5. Figure 4
Transcriptional Regulation of Cyclin and Cyclin/CDK Regulator Genes
(A) Summary of previous evidence for transcriptional regulation of genes encoding the cyclins (green) and cyclin/CDK regulators (red) by the cell cycle transcription factors (Althoefer et al., 1995; Dirick et al., 1992; Hollenhorst et al., 2000; Iyer et al., 2001; Knapp et al., 1996; Koch et al., 1993, 1996; Koranda et al., 2000; Kumar et al., 2000; Kuo and Grayhack, 1994; Loy et al., 1999; Mackay et al., 2001; McBride et al., 1999; McInerny et al., 1997; Nasmyth and Dirick, 1991; Oehlen et al., 1996; Ogas et al., 1991; Partridge et al., 1997; Pic et al., 2000; Schwob and Nasmyth, 1993; Toyn et al., 1997; Zhu et al., 2000). The factors, as well as their targets, are positioned according to their approximate time of function. The relationships between the transcription factors and their target genes are indicated by arrows, solid lines represent evidence for direct regulation by these factors, and dashed lines represent inferences from indirect evidence.
(B) Model for transcriptional regulation of cyclin and cyclin/CDK regulators based on previous studies and on genome-wide binding data. Each group of transcription factors regulates key cell cycle regulators that are needed for progression through the cell cycle
Cell  , DOI: ( /S (01) )

6. Figure 5 Regulation of Cell Cycle Functions by the Activators
Stage-specific cell cycle functions under the control of specific factors are shown. The budding category includes genes involved in budding and in cell wall biogenesis; the DNA replication category includes genes involved in replication, repair, and sister chromatid cohesion; the chromatin category includes genes encoding histones, chromatin modifiers, and telomere length regulators. The identity and function of genes in each category are listed in Table 1
Cell  , DOI: ( /S (01) )

7. Figure 6 Partial Redundancy between Homologous Activators
(A) Venn diagrams depicting the overlap between the targets of pairs of homologous cell cycle transcriptional regulatory proteins. The numbers in parentheses under each activator represent the sum of cell cycle genes whose promoters were bound by the protein. The number in the intersection between two circles reflects the number of genes whose promoters were bound by both proteins.
(B) Venn diagrams representing the overlap in target sites between pairs of regulatory proteins that reside within the same complex.
(C) A Venn diagram representing the overlap in target sites between two transcriptional regulators that are not known to be related
Cell  , DOI: ( /S (01) )