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Effect of skeletonization of the internal thoracic artery on vessel wall integrity  Mario Gaudino, MD, Amelia Toesca, PhD, Stefania Lucia Nori, MD, Franco.

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Presentation on theme: "Effect of skeletonization of the internal thoracic artery on vessel wall integrity  Mario Gaudino, MD, Amelia Toesca, PhD, Stefania Lucia Nori, MD, Franco."— Presentation transcript:

1 Effect of skeletonization of the internal thoracic artery on vessel wall integrity 
Mario Gaudino, MD, Amelia Toesca, PhD, Stefania Lucia Nori, MD, Franco Glieca, MD, Gianfederico Possati, MD  The Annals of Thoracic Surgery  Volume 68, Issue 5, Pages (November 1999) DOI: /S (99)

2 Fig 1 (A, B). Morphologic light microscopic evaluation of a pedicled mammary graft shows good maintenance of the arterial wall architecture (A = adventitia, E = endothelium, EEL = external elastic lamina, IEL = internal elastic lamina, M = media). The Annals of Thoracic Surgery  , DOI: ( /S (99) )

3 Fig 2 (A, B). Morphologic light microscopic evaluation of a skeletonized mammary graft shows good maintenance of the arterial wall architecture (A = adventitia, E = endothelium, EEL = external elastic lamina, IEL = internal elastic lamina, M = media). The Annals of Thoracic Surgery  , DOI: ( /S (99) )

4 Fig 3 Immunohistochemical staining with anti-factor VIII antibody (stained in brown) demonstrates optimal preservation of the endothelial layer in a pedicled mammary graft (A = 10 ×, B = detail of A 64 ×). The Annals of Thoracic Surgery  , DOI: ( /S (99) )

5 Fig 4 Immunohistochemical staining with anti-factor VIII antibody (stained in brown) demonstrates optimal preservation of the endothelial layer in a skeletonized mammary graft (A = 10 ×, B = detail of A 64 ×). The Annals of Thoracic Surgery  , DOI: ( /S (99) )

6 Fig 5 Morphological electron microscopic evaluation demonstrates good preservation of the ultrastructural aspect of both pedicled (A) and skeletonized (B) mammary graft (BL = basal lamina, E = endothelium). The Annals of Thoracic Surgery  , DOI: ( /S (99) )

7 Fig 6 Electron microscope analysis of a skeletonized mammary graft shows good preservation of the intercellular junction between endothelial cells (G = gap junction). The Annals of Thoracic Surgery  , DOI: ( /S (99) )

8 Fig 7 Electron microscope imaging of a microthrombus in a skeletonized mammary graft (E = endothelium, L = vessel lumen, RBC = red blood cell). The Annals of Thoracic Surgery  , DOI: ( /S (99) )


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