1. mRNAs that show increased protein synthesis following UPF1 depletion are enriched for those with very long 3′UTRs.
mRNAs that show increased protein synthesis following UPF1 depletion are enriched for those with very long 3′UTRs.A, The principal transcripts of the 30 most up- or downregulated and the 30 least regulated genes from the group of significant UPF1 targets were analyzed for the length of the 3′UTR. Sequence information was taken from the Ensembl database. Significantly more up-regulated UPF1 target mRNAs contain a long 3′UTR (>1kb) compared with downregulated targets. Fisher exact test was applied for statistical analysis. B, Same analysis as in (A) with 28 most up- and downregulated from the list of targets that overlap between pSILAC screen and RNA-seq (11). mRNA equivalents of only 28 downregulated proteins were found in the RNAseq, hence also the 28 most up-regulated transcripts were used for the comparison. C, D, Length of the 3′UTRs of the principal transcripts from (A) and (B). Dots indicate the individual length of the 3′UTR, the horizontal bars represent the medians. Significance was analyzed by Mann-Whitney test.
Jana Sieber et al. Mol Cell Proteomics 2016;15:
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.