1. Two-dimensional gel silver staining and two-dimensional immunoblotting using antibody to 3-nitrotyrosine.
Two-dimensional gel silver staining and two-dimensional immunoblotting using antibody to 3-nitrotyrosine. Immunoprecipition of vimentin, desmin, ATP synthase β-chain subunit, protein disulfide-isomerase A3, voltage-dependent anion channel 1 and MnSOD and immunoblotting using antibody to 3-nitrotyrosine. A-Matching immunoreactive spots specifically present in MD patients are squared and numbered in a representative two-dimensional silver-stained gel and in the corresponding anti-3-nitrotyrosine two-dimensional immunoblotting of a patient (P) and a control (C) before and following reduction (AR). The immunopositive spots detected following the reduction were not considered for further analysis. The corresponding proteins were identified as reported in Table III. B-Anti-3-nitrotyrosine two-dimensional immunoblotting following immunoprecipitation of vimentin, desmin, ATP synthase β-chain subunit, protein disulfide-isomerase A3, voltage-dependent anion channel 1, and MnSOD shows expected spots corresponding to proteins.
Gaetano Vattemi et al. Mol Cell Proteomics 2011;10:M
© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.