1. Arabidopsis Thaliana Gene AT5G58610
Nancy Gallardo
HC70AL

2. What Protein Does AT5G58610 Code for?
PHD-Type Zinc Finger Transcription Factor
Regulates gene expression
1065 amino acids

3. What's Around Gene AT5G58610 ? AT5G58600 5' 3' 3' 5' 3' 5' 5' 3'
3' ' ' ' ' '
AT5G58600
AT5G8610
AT5G58620
AT5G58620
5' ' ' ' ' ' 3' 5'
1045bp bp

4. What is the Structure of AT5G58610 ?3' 5' 5' 3'
Exon
Exon
Exon
Exon
Exon
Exon
Exon
Exon
Exon
Chromosome 5
4283 base pairs
Reverse Orientation
9 Exons and 8 Introns

5. Expected T-DNA Insertion Salk_041367
LBb1-150bp
Expected Sizes:
WT= 908bp
T-DNA= 696bp
T-DNA
Rv- 20bp 3' 5'
Intron 2
Intron 1
546bp bp
Exon 1
Fw- 20bp

6. What Were the Genotyping Results ?
WT= T-DNA=696
PCR Genotyping
AT5G58610
700bp
1% agarose, 105 Volts, 04/15/2008, 1.5Hour Run

7. Do these results agree with the RT-PCR results?

8. Where is AT5G58610 Active? RT-PCR AT5G58610 1% Agarose 1 Hour Run
70 Volts
04/24/2008

9. Arabidopsis Thaliana Gene AT4G33280

10. What Protein Does My Gene Code for?
B3-Domain Transcription Factor Families
Regulate gene expression
3 families in Arabidopsis Thaliana
337 amino acids

11. What's Around Gene AT4G33280 ? 5' 3' AT4G33270 AT4G33280 AT4G33290 3'
3' ' '
AT4G33270
AT4G33280
AT4G33290
5' ' ' 3'
561bp
217bp 5'

12. What is the Structure of AT4G33280?3' 5' 5' 3'
UTR
Exon
Exon
Exon
Exon
Exon
UTR
Intron Intron Intron Intron
Chromosome 4
2002 Base pairs
Reverse Orientation
5 Exons, 4 Introns, 2 UTRs

13. Expected T-DNA Insertion
Salk_072801
Expected Sizes:
WT= 402bp
T-DNA= 292bp
LBb1-150bp
T-DNA
Rv-20bp 5' 3'
AT4G32270
260Bp bp
Intron 4
Exon 5
Fw-23bp

14. What Were the Plants' Genotypes ?
Expected T-DNA=292bp Expected Wild Type=402bp
PCR Genotyping AT4G33280
Wild Type= 25
Homo T-DNA= 0
Hemi T-DNA= 0
1% agarose
1 Hour
05/15/2008
105 Volts

15. Carrying Out More Genotyping
PCR Genotyping AT4G33280
1% agarose
05/29/08
1Hr. Run
105 Volts

16. Genotyping With Controls
PCR Genotyping of Plants: 7, 17, 26, 33
1% Gel
05/30/08
1 Hr.Run
105 Volts

17. AT4G33280's Activity RT-PCR AT4G33280 Expected Product= 254bp
5:34-7:12 pm
1.5% Agarose
05/20/2008
105 Volts
Expected Product= bp

19. Cloning the Upstream Region
Colony Digestion
pENTR+ Gene
Vector Promoter
Non-Recombinant bp bp
Recombinant bp
1% Agarose, 1 Hour Run, 1% agarose, 105 Volts

20. Nomarski

21. THANKS TO: Dr. Goldberg Anhthu Bui Bekah Charney Daisy Robinton
Brandon Le
Tomokazu Kawashima