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Living, autologous pulmonary artery conduits tissue engineered from human umbilical cord cells  Simon P Hoerstrup, MD, Alexander Kadner, MD, Christian.

Published byŁucja Urbańska Modified over 5 years ago

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Presentation on theme: "Living, autologous pulmonary artery conduits tissue engineered from human umbilical cord cells  Simon P Hoerstrup, MD, Alexander Kadner, MD, Christian."— Presentation transcript:

1 Living, autologous pulmonary artery conduits tissue engineered from human umbilical cord cells 
Simon P Hoerstrup, MD, Alexander Kadner, MD, Christian Breymann, MD, Christine F Maurus, MD, Christina I Guenter, MD, Ralf Sodian, MD, Jeroen F Visjager, PhD, Gregor Zund, MD, Marko I Turina, MD  The Annals of Thoracic Surgery  Volume 74, Issue 1, Pages (July 2002) DOI: /S (02)

2 Fig 1 Two views (A and B) of tissue engineered pulmonary artery conduit after 14 days conditioning in the pulse duplicator bioreactor (dimensions: length, 40 mm; inner diameter, 18 mm; wall thickness, 1 mm). The Annals of Thoracic Surgery  , 46-52DOI: ( /S (02) )

3 Fig 2 (A) Hematoxylin-eosin and (B) Masson’s trichrome staining showed cells with a fibroblast morphology and deposition of extracellular matrix proteins. Immunofluorescence staining demonstrated expression of (C) vimentin and (D) alpha-smooth muscle actin. The Annals of Thoracic Surgery  , 46-52DOI: ( /S (02) )

4 Fig 3 (A) Hemotoxylin and eosin and Masson’s trichrome staining of the conduit constructs demonstrates cellular tissue organized in a layered fashion and formation of extracellular matrix predominantly comprising glycosaminoglycans. (B) Static controls show a loose, less organized tissue formation with irregular cellular ingrowth. Immunohistochemistry shows positive staining for (C) collagen type I, (D) collagen type III, (E) alpha-smooth muscle actin, and (F) vimentin. The Annals of Thoracic Surgery  , 46-52DOI: ( /S (02) )

5 Fig 4 (A) Scanning electron microscopy of the pulsed conduits showed dense tissue formation and a confluent smooth surface. (B) In contrast, static controls were less homogeneous. (C) Transmission electron microscopy revealed cell elements typical of secretionally active myofibroblasts such as collagen fibrils (asterisk) and elastin (white arrow). The Annals of Thoracic Surgery  , 46-52DOI: ( /S (02) )

6 Fig 5 Mechanical properties graphically displayed as a typical stress-strain curve of each of the investigated materials recorded at room temperature with a linear strain rate of 1 minute−1 of tissue-engineered (TE) pulmonary conduits (red), static controls (blue), and human pulmonary artery (Hum. PA) tissues (black). The Annals of Thoracic Surgery  , 46-52DOI: ( /S (02) )

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