1. Volume 11, Issue 2, Pages 173-183 (February 2004)
In Vitro and In Vivo Production of New Aminocoumarins by a Combined Biochemical, Genetic, and Synthetic Approach 
Ute Galm, Marco Aurélio Dessoy, Jürgen Schmidt, Ludger A. Wessjohann, Lutz Heide 
Chemistry & Biology 
Volume 11, Issue 2, Pages (February 2004)
DOI: /j.chembiol

2. Figure 1
Structures of the Aminocoumarin Antibiotics Clorobiocin, Novobiocin, and Coumermycin A1
The acyl components involved in amide bond formation are indicated by boxes.
Chemistry & Biology  , DOI: ( /j.chembiol )

3. Figure 2
Purification of the Amide Synthetases NovL, CloL, and CouL after Overexpression as a Fusion Protein with a C-terminal His6 Tag in E. coli XL1 Blue MRF′
SDS-PAGE was carried out as described in Experimental Procedures. Lanes 1, molecular weight (MW) marker; lanes 2, total protein before induction; lanes 3, total protein after IPTG induction; lanes 4, soluble protein after IPTG induction; lanes 5, eluate from Ni2+ affinity chromatography.
Chemistry & Biology  , DOI: ( /j.chembiol )

4. Figure 3
Relative Activities of the Amide Synthetases NovL, CloL, and CouL with Different Carboxylic Acids
Specific activities with the genuine substrates of the enzymes were set as 100%.
Chemistry & Biology  , DOI: ( /j.chembiol )