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Gabrielle R. Wilson, B. Sc. (Hons. ), Marcus L. -J. Sim, B. Sc. (Hons

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Presentation on theme: "Gabrielle R. Wilson, B. Sc. (Hons. ), Marcus L. -J. Sim, B. Sc. (Hons"— Presentation transcript:

1 Molecular analysis of the PArkin co-regulated gene and association with male infertility 
Gabrielle R. Wilson, B.Sc.(Hons.), Marcus L.-J. Sim, B.Sc.(Hons.), Kate M. Brody, B.Sc.(Hons.), Juliet M. Taylor, Ph.D., Robert I. McLachlan, Ph.D., Moira K. O'Bryan, Ph.D., Martin B. Delatycki, Ph.D., Paul J. Lockhart, Ph.D.  Fertility and Sterility  Volume 93, Issue 7, Pages (May 2010) DOI: /j.fertnstert Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Alignment of the AluJ consensus sequence (reverse complement) and NM_ PACRG exon 5. The predicted exon is shown in uppercase type and boxed, the flanking intronic sequence is shown in lowercase italic type, and the predicted splice acceptor/donor consensus sequences are indicated in boldface type. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Analysis of the structure of the PACRG transcript in human testis. Agarose gel electrophoresis of reverse-transcription polymerase chain reaction amplification of PACRG. The amplification products using primers corresponding to both NM_ and AF_ (set 1 to set 8) are shown. The final three lanes use primer sequences specific for exon 5 of NM_ (set 9 to set 11). The fragment sizes of the DNA molecular weight marker are indicated in basepairs. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions


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