1. Fully differentiated human colon cancer T84 cell line expressing the structural and functional characteristics of colonic cells.
Fully differentiated human colon cancer T84 cell line expressing the structural and functional characteristics of colonic cells. (A) Scanning electron microscopy micrograph shows the well-organized brush border with short microvilli. (B and C) Transmission electron microscopy micrograph shows the polarized organization of the cells forming a monolayer and the apical brush border composed of short microvilli. Arrows indicate TJs. (D) Confocal laser scanning microscopy micrograph shows the mosaic pattern of expression of immunolabeled functional brush border-associated dipeptidyl-peptidase IV (DPP IV) (x-y section). (E) Confocal laser scanning microscopy examination of immunofluorescence labeling of TJ-associated occludin protein shows the expression at the cell-to-cell contact of confluent cells (x-y section). Confluent cells at 15 days in culture are shown. (Panels A and C courtesy of P. Hofman, reproduced with permission.)‏
Vanessa Liévin-Le Moal, and Alain L. Servin Microbiol. Mol. Biol. Rev. 2013; doi: /MMBR