1. 4/15 Daily Catalyst Pg. 53 Gel Electrophoresis
1. Why do insertions and deletion mutations have a more devastating affect?
2. Why do women need to be cautious around potential mutagens?
3. Determine the mutation:
DNA: ACG- TAC-GGC
Mut: AGG-ACG -GGT

2. 4/15 Class Business Pg. 53 Gel Electrophoresis
Quiz #12 DNA on Friday
DNA test 4/21
Study guide due test
Review day on 4/20
DNA Extraction lab on Thursday
Be here! Labs are test grades!
Interims come out this Friday
Signed for extra credit
Parent Night on Thursday 6-7:30
Extra Credit

3. 4/15 Agenda Pg. 53 Gel Electrophoresis
Daily Catalyst
Class Business
Gel Electrophoresis notes
Project work time
Exit Ticket #9 Mutations
Homework: finish the gel electrophoresis packet and DNA packet pg. 5

4. 4/15 Objective Genetic engineering techniques can manipulate the heritable information of DNA by gel electrophoresis.

5. Video Clip Gel Electrophoresis video clip

6. Genetic Engineering Key Point #1: Genetic engineering (GE)
Scientific alteration of the DNA (genetic material)
Transferring genes between organisms or modifying the genes in an organism to remove or add a desired trait
For example: Genetic engineering has been employed to create bacteria that synthesize insulin and other human proteins.

7. Gel Electrophoresis Key Point #2: Gel Electrophoresis
The separation of DNA based on positive and negative charges
What is DNA’s charge?
DNA is negatively charged
How?
Negative phosphate groups

8. How Gel Electrophoresis works
DNA molecules are added to a gel
DNA molecules move down through the gel
Key Point #3: Molecules move based on their size
Smaller molecules move faster through the gel

9. Key Point #4: Molecules move from the negative to the positive side of the gel

10. Why are we learning about this?
Every living cell has DNA
Every living cell has unique DNA
Key Point #5: DNA is like our fingerprint, unique to us and can link us to the scene of a crime
Gel electrophoresis has only been used since 1980’s
This technology has revolutionized the way crimes are solved.

11. How does this process work?
DNA is collected from the crime scene and from potential suspects
DNA is dropped into individual columns
The gel is connected to a power source (+ and -)
The DNA moves down the gel
Similar DNA will show the same patterns and the patterns are matched to the suspect’s.

12. Example

13. Crime video clip

14. Project
Directions: Today, you and a partner will read several gels and determine if the suspect is guilty or not. When you finish the gel project, prepare for exit ticket.
Time: 18 minutes
Noise: 1 (with partner)

15. Exit Ticket #9 Mutations
Name: ___________ Date: 4/15 Period: 1 Score ______/4
1. Define mutations.
2. Are all mutations bad?
3. Mutations can lead to an increase in ______________.
4. What causes mutations?

16. Exit Ticket #9 Mutations
Name: ___________ Date: 4/15 Period: 1 Score ______/4
1. Define mutations.
A random change in DNA
2. Are all mutations bad?
NO, some are helpful
3. Mutations can lead to an increase in ______________.
Variation
4. What causes mutations?
Mutagens