1. Tomato FISH
Song-Bin Chang
Suzanne Royer
Lorrie Anderson
Steve Stack

2. in Sequencing the Tomato Genome
Functions of FISH
in Sequencing the Tomato Genome
1. Confirming the locations of BACs
2. Demonstrating the order of BACs
3. Determining the distance between BACs
4. Locating problem BACs
5. Determining the size of gaps between contigs

7. BAC 116C14, Slide 126, Short (p) Arm, Chromosome 9

12. * * * * * *
Short arm
Centromere
Long arm
Japan
Spain
Italy
euchromatin
heterochromatin
AT-rich satellite DNA Mb 1 10 11 12 9
USA UK
China
Korea
France 6 2 3 4 7
The Netherlands 5 8 17 16 10 13 11 24 26 19 20 27
India

13. 45 BACs FISHED SO FAR 15 gave no FISH signal.
5 had too many repeated signals.
25 were successfully localized.

14. Number of BACs localized
so far

15. SOL Genomics Network

16. SOL Genomics Network

17. Chromosome Research Send your next cytogenetics manuscript to
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