1. Estrogen receptor β agonist diarylpropionitrile inhibits lipopolysaccharide-induced regulated on activation normal T cell expressed and secreted (RANTES) production in macrophages by repressing nuclear factor κB activation 
Shi-ying Huang, M.S., Hong Xin, M.D., Jing Sun, M.D., Rui Li, M.S., Xue-mei Zhang, Ph.D., Dong Zhao, M.D. 
Fertility and Sterility 
Volume 100, Issue 1, Pages (July 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
ERβ expression in RAW264.7 cells. (A) RAW264.7 and mouse ovary lysates were analyzed by RT-PCR for the presence of the mRNA encoding ERβ. (B) ERβ protein expression in mouse ovary, murine PMΦ, and RAW264.7 (RAW) lysates using Western blot analysis.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Effect of DPN on RANTES production in murine RAW264.7 and RAW264.7 after ERβ siRNA transfection. (A) RANTES production was determined after a 2-hour pretreatment with saline, DPN, or dexamethasone (positive control), followed by treatment for 24 hours with LPS. (B) RANTES level after siERβ transfection. Nontargeting siRNA [RNAi(−)] was set as the negative control. RAW264.7 cells were pretreated with 10−7 mol/L DPN or saline, and after 24 hours LPS stimulation. The data reported are the mean ± SEM of three independent experiments, each performed in duplicate. *P<.05 as compared with the control group. (C) ERβ protein level in RAW264.7 after ERβ siRNA transfection.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
DPN suppresses p65 phosphorylation and IκB degradation in RAW (A) Effects of NF-κB signal on RANTES accumulation. Cells were pretreated for 2 hours with DPN (10−7 mol/L) or PDTC (10−5 mol/L) and then were stimulated with LPS for 24 hours. *P<.05 as compared with the control group. #P<.05 as compared with the LPS group. (B) Effect of DPN on LPS-induced NF-κB activation and p65 phosphorylation in time course. (C) Effect of DPN on IκB degradation in time course. (D, E) Quantitative data depicting the phospho-p65/total p65 ratio (D) and the IκB/β-actin ratio (E). Cells were pretreated for 2 hours with or without DPN (10−7 mol/L) and then were stimulated with LPS (1 μg/mL) for 0, 5, 10, 15, 30, and 60 minutes. The protein levels were quantitated by mean density value. The results are expressed as the mean ± SEM of four independent experiments, each performed in triplicate. *P<.05 compared with the LPS group at each respective time point.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
DPN suppressed NF-κB nuclear translocation in RAW (A) RAW264.7 cells were grown on glass coverslips and [1] left untreated, [2] treated with LPS for 15 minutes, or [3] pretreated with DPN for 2 hours, followed by 15 minutes of LPS stimulation. Cells were visualized using a fluorescent microscope (scale bar = 20 μm). (B) The relative intensity (nucleus/cytoplasm) of the p65 fluorescence in panel A was quantified. Over 80 cells were analyzed in each case. The results are expressed as the mean ± SEM of four independent experiments. *P<.05 compared with the control group. #P<.05 as compared with the LPS group.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions