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Testing for Maternal Cell Contamination in Prenatal Samples

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Presentation on theme: "Testing for Maternal Cell Contamination in Prenatal Samples"— Presentation transcript:

1 Testing for Maternal Cell Contamination in Prenatal Samples
Iris Schrijver, Sarah C. Cherny, James L. Zehnder  The Journal of Molecular Diagnostics  Volume 9, Issue 3, Pages (July 2007) DOI: /jmoldx Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Clinical practice questionnaire, used in phone interviews.
The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Distribution of accepted specimen types in 35 molecular diagnostic laboratories. DA, direct amniotic fluid; CA, cultured amniotic fluid; DCVS, direct chorionic villus sample; CCVS, cultured chorionic villus sample. The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Sample requirements. a: Tissue sample requirements for direct CVS testing (n = 21 laboratories). b: Sample volume requirements for direct AF testing (n = 21 laboratories). The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 MCC testing practice for 34 US laboratories. Four laboratories never test for MCC, nine perform MCC testing on occasion, six performed it whenever a maternal sample was available, and 15 performed it without exception. The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

6 Figure 5 The reported lower limit of detection for MCC in US diagnostic laboratories. The lower limit of detection is indicated in the legend on the right and by the columns of the histogram, whereas the number of laboratories for each category is listed on top of the columns. Each individual column represents a reported sensitivity category of the MCC assays in use. This question was answered by 27 laboratories. Individual methods for determination of the lower limit of detection were not disclosed. The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

7 Figure 6 A practical testing algorithm for prenatal samples.
The Journal of Molecular Diagnostics 2007 9, DOI: ( /jmoldx ) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions


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