1. THE NQAD IS HIGHLY LINEAR, EVEN AT LOW CONCENTRATIONS WHICH ARE UNDETECTABLE BY THE ELSD
Application note # QAN-0090 ™
APPLICATION NOTES
CHROMATOGRAMS
FLOW RATE: 1.00 mL/min
COLUMN: Phenomenex Kinetex C18, 150 x 4.6, 2.6 u
COLUMN TEMP: Ambient
INJECTION VOLUME :1 µL
ANALYTE: Tetracycline
NQAD
GAS: Air
FILTER: 1.3 sec
GAIN: 1X
EVAPORATION TEMP: 35C
ELSD
GAS: Nitrogen, 1.5 L/min
GAIN: 1
EVAPORATION TEMP: 40C
HPLC
MOBILE PHASE: ACN:Water with 0.2% TFA
PARAMETERS
Demonstrates better linearity than ELSD
QUANT TECHNOLOGIES, LLC
TH Lane NE
Blaine, MN, USA
Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s. 1