1. Quantification of the effects of transport on population changes in both transport and cargo strains in the presence and absence of Amp.
Quantification of the effects of transport on population changes in both transport and cargo strains in the presence and absence of Amp. Selective viable counts (black bars, E. coli; white, P. vortex) were used to determine transporter and cargo CFU after inoculation on 14-cm-diameter MH agar plates with transporter (P. vortex [PV]) and/or cargo (E. coli [EC]) bacteria, with the change in viable count assessed by selective plating after 72 h at 37°C. PNPG, addition of the swarming inhibitor p-nitrophenylglycerol (0.5 mM). The broken horizontal line indicates the inoculum level (5 × 107 CFU) for each species. (a) Plates without antibiotic; (b) plates with 200 µg ml−1 Amp.
Alin Finkelshtein et al. mBio 2015; doi: /mBio