1. The TRAF-Interacting Protein (TRIP) Is a Regulator of Keratinocyte Proliferation 
Stéphanie Almeida, Stephan Ryser, Magdalena Obarzanek-Fojt, Daniel Hohl, Marcel Huber 
Journal of Investigative Dermatology 
Volume 131, Issue 2, Pages (January 2011)
DOI: /jid
Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
TRAF-interacting protein (TRIP) mRNA expression is repressed by keratinocyte differentiation or cell growth arrest. (a) Change in expression levels of TRIP and keratin 1 (HK1) mRNAs in response to increasing cell density or (b) to increasing calcium concentration in normal human foreskin keratinocytes (NHEKs). (c) Time course of TRIP mRNA level in NHEKs after phorbol-12-myristate-13-acetate (TPA; 100nM, 2hours) treatment (white columns) compared with vehicle-treated cells (black columns). (d) TRIP mRNA level in NHEKs at 24hours after treatment with TPA (white columns) compared with vehicle-treated cells (black columns) in the presence or absence of 10μM GF109203X. The results are shown as mean±SD from three (a, b, d) or six (c) independent experiments. **P<0.01, ***P<0.001 versus day 0 (a, b) or vehicle-treated cells (c, d).
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
TRAF-interacting protein (TRIP) expression is suppressed by inhibition of the phosphatidylinositol-3 kinase (PI3K)/Akt signaling pathway. (a) Quantitative reverse transcriptase PCR (RT-PCR) analysis of TRIP expression in normal human foreskin keratinocytes (NHEKs) cultured in low-calcium medium after treatment with different inhibitors (20μM PD98059, 10μM GF109203X, 5μM SP600125, 50μM LY294002, 10μM SB203580, 10μM SB202190, and 5μM AG1478) for 24hours. Results are depicted as mean±SD from two (PD98059, GF109203X, SP600125) or three (all other inhibitors) independent experiments. (b) Effect on TRIP expression in NHEKs following rapamycin treatment for 24hours. Results are shown as mean±SD from three independent experiments. **P<0.01 versus control vehicle-treated cells.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
TRAF-interacting protein (TRIP) knockdown in normal human foreskin keratinocytes (NHEKs) leads to cell growth arrest and blocks cells in G1/S phase. (a) BrdU incorporation (mean±SEM, n=3) in T4 and T5-KD cells relative to control A. (b) Cell cycle distribution in T4 (white) and T5 (grey)-KD NHEKs compared with control A short-hairpin RNA (shRNA; black) at 96hours postinfection (left panel) and comparison of G1/S ratios (right panel). Results (mean±SEM) are from one experiment performed in triplicates. Similar results were obtained in three independent experiments. (c) Immunoblot analysis of CDK4 and CDK6 (left panel) and Rb (right panel) expression in TRIP-KD NHEKs. (d) Flow cytometry analysis (mean±SEM; n=3, 48 and 72hours; n=2, 96hours) for viable, apoptotic, and necrotic NHEKs at 48 (black), 72 (white), and 96hours (grey) postinfection. *P<0.05, **P<0.01, ***P<0.001 versus control.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
TRAF-interacting protein (TRIP) knockdown in normal human foreskin keratinocytes (NHEKs) induces differentiation marker expression but not NF-κB activity. (a) Quantitative reverse transcriptase PCR (RT-PCR) analysis of keratin 5, keratin 1, and filaggrin gene expression 4 days after infection with the indicated lentivirus (LV). Results are reported as mean±SEM from 4 to 6 independent experiments. *P<0.05, **P<0.01 versus control short-hairpin RNA (shRNA). (b) Ratio of luciferase activities that were measured at 72 and 24hours post-transduction in NHEKs infected with the indicated LV (left panel). The middle and right panels depict tumor necrosis factor-α (TNF-α)-mediated induction of luciferase reporter activity directed by the NF-κB promoter and the minimal cytomegalovirus (CMV) promoter, respectively. Cells were incubated with TNF-α at the indicated concentrations for 2hours. Results are depicted as mean±SD from two independent experiments.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
TRAF-interacting protein (TRIP) expression is increased in basal cell carcinoma (BCC). Relative mRNA expression levels of (a) Gli1 and (b) TRIP are increased in BCCs (B) compared with normal skin (N). Relative expression levels were calculated using RPL13A as endogenous control gene for each individual biopsy of normal skin (n=5) and BCC (n=5).
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions