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Diagnosis of Trypanosomatid Infections

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Presentation on theme: "Diagnosis of Trypanosomatid Infections"— Presentation transcript:

1 Diagnosis of Trypanosomatid Infections
Pablo González-Andrade, Mamady Camara, Hamidou Ilboudo, Bruno Bucheton, Vincent Jamonneau, Stijn Deborggraeve  The Journal of Molecular Diagnostics  Volume 16, Issue 4, Pages (July 2014) DOI: /j.jmoldx Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Melting peak of reverse-transcribed and PCR-amplified SL-RNA in total RNA from 2 million T.b. gambiense parasites. Non-reverse-transcribed (NRT) total RNA was used as a negative control. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Amplification curves and melting peaks (inset) of the SL-RNA in 10-fold serial dilutions of T.b. gambiense parasites in human blood. Expressed as parasites per milliliter of blood. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Melting peaks of reverse-transcribed and PCR-amplified total RNA (10 pg) from different trypanosomatids. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions


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