1. c16000 Line Balancing July 2007 Document Control Number 204129-101
©2007, Abbott Laboratories,
Abbott Park, Illinois

2. c16000 Line Balancing Document Control Number Revision or Release Date
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Revision or Release Date
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07/2007
New Release
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

3. What is Line Balancing? R2 R1
The c16000 has two R1 reagent probes and two R2 reagent probes. The dual probes allow the c16000 to process up to twice as many photometric tests as the c8000. This occurs because the probes can simultaneously aspirate reagents for two different assays in each 4.5 second cycle.
Each pair of probes has an A-line probe that can only access the outer ring (segments A, B, and C) of the carousel and a B-line probe that can only access the inner ring (segment D) of the carousel.
Each probe then dispenses into the corresponding A-line or B-line cuvette of each cuvette pair.
Outer ring A-line R2
Inner ring B-line R1
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

4. Why is Line Balancing Important?
If reagents are loaded optimally between the Outer (A-line) and Inner (B-line) carousels, the c16000 will fill cuvettes more efficiently and maximize throughput.
If reagents for a common panel are loaded more heavily on one line versus the other, the system will leave empty cuvettes and the throughput is decreased.
Line balancing involves more than ensuring an even number of reagents are loaded on the inner and outer carousels. The example on the next page demonstrates the impact of uneven line balancing.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

5. Line Balancing Example
In the following example, there are two panels that are frequently ordered:
Panel 1 – Na, K, Cl, CO2, Glu, Ca, Urea, Creat, TP, and Alb
Panel 2 – Alb, AST, ALT, T Bili, and AlkP
Here are two different ways you could load the reagent carousels
Example 1
Example 2
Reagents #
A-line (Outer)
ICT, Glu, Urea, TP, T Bili, AST, AlkP 7
B-line (Inner)
CO2, Ca, Alb, Creat, ALT 5
Reagents #
A-line (Outer)
ICT, Glu, CO2, Urea. Creat, Ca 6
B-line (Inner)
T Bili, Alb, AST, ALT, AlkP, TP
It would seem that the reagents are more balanced in Example 2. Is this confirmed when the system dispenses the tests for the frequently ordered panels?
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

6. Line Balancing Example
Frequently ordered panels:
Panel 1 – Na, K, Cl, CO2, Glu, Ca, Urea, Creat, TP, and Alb
Panel 2 – Alb, AST, ALT, T Bili, and AlkP
Reagent Loading Example 1
Reagent Loading Example 2
Reagents #
A-line (Outer)
ICT, Glu, Urea, TP, AST, T Bili, AlkP 7
B-line (Inner)
CO2, Ca, Creat, Alb, ALT 5
Reagents #
A-line (Outer)
ICT, CO2, Glu, Ca , Urea, Creat 6
B-line (Inner)
TP, Alb, AST, ALT, T Bili, AlkP
System Dispense Results
System Dispense Results
Panel 1
Panel 2
Panel 1
Panel 2
Cuvettes
A-line
B-line
ICT
Gluc
CO2
Urea Ca TP
Alb
Creat
Cuvettes
A-line
B-line
T Bili
AST
ALT
AlkP
Alb
Cuvettes
A-line
B-line
ICT
CO2 TP
Glu
Alb Ca
Urea
Creat
Cuvettes
A-line
B-line
Alb
AST
ALT
T Bili
AlkP
Cycles = 3
Empty Cuv. = 1
Cycles = 5
Empty Cuv. = 2
Cycles = 5
Empty Cuv. = 5
Cycles = 6
Empty Cuv. = 4
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

7. Line Balancing Example
As you can see from the previous pages, with the reagents loaded as in Example 1, the system generated fewer empty cuvettes and needed fewer cycles to run the assays Leading to a FASTER THROUGHPUT!!!
Now it’s time to maximize your customer’s throughput.
Use the reagent configuration shown on pages 8 and 9 as a starting point.
This configuration is based on commonly ordered chemistry panels and tests
To further optimize the configuration for a specific customer’s assay menu, you can use the detailed instructions on pages
Information required to perform the analysis:
The most frequently ordered panels and/or assays for the lab
The menu of assays that will be run on the c16000
Bonus – the assay package inserts allow you to further refine the line balancing by reducing the need for SmartWashes.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

8. Typical Arrangement – Reagent Carousel View
The following is an example of how you might load the reagent carousels for a typical laboratory work list.
NOTE: Some assays are available outside the US only. The illustration above is provided as an example and is intended for informational use only.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

9. Typical Arrangement List
A-Line (Outer)
Detergent A
10% Dtg B
0.5% Acid Wash
Saline (dilution)
Water Bath Additive
ICT Sample Diluent
Glucose
Urea
Total Protein
AST
Total Bilirubin
Lipase
Phosphorus
GGT LD
Neonatal Bilirubin
HDL-Ultra
Amylase
ApoA
ApoB C3 C4
IgA
IgM
IgG
Prealbumin RF
Transferrin
Haptoglobin
Microalbumin
CRP
Iron
Lactic Acid
Acid Phos
Saline-HIL
A -1-Acid Glycoprotein
A -1-Antitrypsin
Amylase (pancreatic)
ASO
b-2-Microglobulin
Bile Acids
Ceruloplasmin
Cholinesterase
"Cholinesterase (Dibucaine) "
CK-MB
Copper
D-Dimer
Digitoxin
Ferritin
Fructosamine
HBDH
IgE
K Light Chains
λ Light Chains
Lp(a)
Myoglobin
B-Line (Inner)
Detergent A
10% Dtg B
0.5% Acid Wash
Saline (dilution, R1 only)
CO2
Calcium
Creat
AlbG/AlbP
ALT
Alk Phos
Triglyceride
Cholesterol
Direct LDL
Uric
Direct Bilirubin
Urine Protein
Magnesium CK
UIBC
Ammonia
NOTE: Some assays are available outside the US only. The table above is provided as an example and is intended for informational use only.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

10. Detailed Line Balancing Instructions
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

11. Line Balancing Instructions – Atypical Assays
The following assays require special processing:
The ICT assay always dispenses in the A-line (outer). The system cannot use the B-line cuvette in the same cycle. Therefore the B-line cuvette remains empty when an ICT test is processed.
When an assay requires a sample volume greater than 8 µL, the system cannot aspirate and dispense sample into both the A-Line and B-Line cuvettes in the same cycle. These assays, (such as Creatinine and Iron), can be loaded in either the A-Line (outer) or B-Line (inner) reagent carousels. However, when these assays are dispensed the corresponding B-Line or A-Line cuvette remains empty.
When an assay requires an onboard dilution, the system cannot aspirate and dispense sample into both the A-Line and B-Line cuvettes in the same cycle. These assays, (such as IgA and IgM), can be loaded in either the A-Line (outer) or B-line (inner) reagent carousels. However, when these assays are dispensed, the corresponding B-Line or A-line cuvette remains empty.
Although these atypical assays may impact system throughput, they should not be evaluated when determining if the A and B lines are evenly balanced.
The following example illustrates how the system leaves some cuvettes empty when running these atypical assays.
Cuvettes
A-line
B-line
ICT
empty
Gluc
CO2
Urea Ca TP
Alb
Creat
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

12. Line Balancing Instructions – Step 1
Wash Solution and Diluents
Load all the wash solutions on both the A-line and B-line
Load the saline used for sample dilution on both the A-line and B-line (R1 only)
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

13. Line Balancing Instructions – Step 2
Determine if the customer is running Lipase?
There are several reagents that significantly interfere with lipase and require SmartWashes. Placing these assays on opposite lines minimizes the potential for interference and reduces the need for extra SmartWashes.
Use the assays from the Lipase package insert that require line separation on the AEROSET System as a guideline for configuring the c16000.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

14. Line Balancing Instructions – Step 3
Identify the most frequently ordered panels
Distribute the assays from the frequently ordered panels between the two lines.
You may have to move assays several times to ensure you have the best balance for the frequently ordered tests.
Remember ICT and Creat are atypical assays that are not evaluated when determining line balancing.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

15. Line Balancing Instructions – Step 4
Distribute individual test(s)
Individual tests that are ordered frequently but are not part of any panels, are distributed evenly in the remaining positions.
Once you have filled the 20 positions of the B-line (Inner) carousel, the remaining tests are loaded in the A-line (Outer) carousel.
These should be the less commonly ordered assays.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

16. Line Balancing Instructions – Step 5
To further refine line balancing, review the reagent SmartWash definitions in the assay package inserts.
A SmartWash is defined when one reagent interferes with another.
If these reagents are loaded on opposite lines, the system never pipettes the reagents from the same probe so the SmartWash is never required.
Line separation of assays requiring a wash reduces the amount of wash solution used and increases throughput.
If you move reagents to eliminate washing, ensure your common panels remain balanced.
The distribution of common tests is more important than eliminating a few SmartWashes because the system will rearrange the aspiration order of tests to avoid performing a SmartWash.
Document Control Number ©2007, Abbott Laboratories,
Abbott Park, Illinois
Company Confidential Not for customer distribution

17. EOD