1. Program

2. Genetics Epigenetics Environmental «triggers» You Nutrition Lifestyle Stress Infections Microbiota ++++ Interactions

3. Neuropeptides in urine LC- MS/MS Dag Tveiten Adrian Finvold 2013

4. Peptides to nervous system Incomplete breakdown of gluten and casein Permeability – Leaky gut syndrome Pathway Diet and effect

5. UV- spectrum of urine UV-spectrum of urine with given wavelengths

6. Derek - 27 Difficult birth – normal weight Reduced weight soon after birth 9,5 kg when 2,5 years old Diagnosed with Infantile Autism - 4 years old Often GI symptoms – pain, diarea Angry often/aggressive behaviour

7. Derek Derek 2005 – 50 kg Derek 2007 – 64 kg ? With permission from parents

8. Derek Before GFCF diet: Rigid, stiffness, unrelaxed Hardly no eye contact Aggressive Tramping with his feet Biting and kicking After GFCF diet: More relaxed Smiling more often More happy Eyecontact More communicative Parents say: One hour after ingestion of forbidden food – marked increased aggressive behaviour Gluten- and casein free diet

11. Publications

12. Project LC-MSMS Autumn 2011 Investment Ms Cooperation with HIOA, RH (Medinova) 4 students Bachelor in Chemistry

13. Peptide structures Casomorphine 1-4Gluten exorphine C - Mass: 522,2 g/mol- Mass: 591.3 g/mol

14. Enzymatic degrading effects

15. Calibration curve Relation between signal and concentration

16. Mass spectrometry Conclusions Fast Selective Sensitive Reliable

17. Mass spectrometry Patient - LC-MsMs ID/RID Reel krea. Kons. (mmol/L) Kasomorfin 1-3 Kasomorfin 1-4 Kasomorfin 1-4A Kasomorfin 1-5 Kasomorfin 1-5A Kasomorfin 1-7Kasomorfin 1-8 Gluteneksorfin A4 Gluteneksorfin A5 Gluteneksorfin B4 Gluteneksorfin B5 Gluteneksorfin C 2630513,7239,7139,11269,7307,54807,1110,1000000

18. Mass spectrometry Patient

19. Patient example Patient lab results – Antibodies/Peptides IgG cowmilk68 U/mL (<20) IgG casein40 U/mL (<20) IgA casein13 U/mL (<12) Casomorphin 1-441,4 nmol/L/kreatinin Casomorphin 1-545,2 nmol/L/kreatinin Casomorphin 1-7200,1 nmol/L/kreatinin Casomorphin 1-8145,2 nmol/L/kreatinin

20. Hurra.hurra Det er bra.bra.bra. Dette går veien Som tosken og skreien. VH K Vi får ta en titt På det som er blitt Et enigma i en gåte Som nålen i en såte. Hi,hi Kalle Hipp hurra Dette var bra. Nå har vi seiret, Og dette må feires VH kalle den nå helgale E-mails from Kalle