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Chadrick E Denlinger, MD, Michael D Keller, BS, Marty W Mayo, PhD, R

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Presentation on theme: "Chadrick E Denlinger, MD, Michael D Keller, BS, Marty W Mayo, PhD, R"— Presentation transcript:

1 Combined proteasome and histone deacetylase inhibition in non–small cell lung cancer 
Chadrick E Denlinger, MD, Michael D Keller, BS, Marty W Mayo, PhD, R.Michael Broad, PhD, David R Jones, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 127, Issue 4, Pages (April 2004) DOI: /S (03)

2 Figure 1 A, NSCLC cells were treated with butyrate ( μmol/L) for 24 hours. Cell viability was determined on the basis of crystal violet uptake. B, NSCLC cells were incubated in the presence or absence of butyrate (500 μmol/L), and nuclear HDAC activity was determined. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

3 Figure 2 A and B, NSCLC cells were transfected with either the 3x-κB reporter gene or cotransfected with Gal4-p65 and the Gal4-luciferase reporter gene and treated with nothing, butyrate, bortezomib (bortz), or butyrate and bortezomib (*P = .04). C, A549 cells were similarly treated, and Northern blot analysis for IL8 was performed. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

4 Figure 3 A, H358, H460, and A549 cells were treated with nothing, bortezomib (bortz), bortezomib and butyrate, or butyrate alone. Western blots were performed with antibodies for p21, p53, and β-tubulin. B, NSCLC cells were treated as noted above and analyzed by means of FACS analysis. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

5 Figure 4 NSCLC cells were treated with nothing, butyrate, bortezomib (bortz), or combined butyrate and bortezomib. Cell survival was determined by means of crystal violet assay (*P = .01, †P = .06). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

6 Figure 5 A, NSCLC cells were treated with nothing, sodium butyrate, bortezomib (bortz), or butyrate and bortezomib. Apoptosis was assessed and quantified by means of DNA fragmentation (A, *P = .02), caspase-3 activation (B, *P = .03), and flow cytometry (C). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

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