1. The 2015 CryoEM Map and Model Validation Challenges

2. 17-Jun-2015
2980 EMDB map entries
842 PDB EM coordinate entries

3. EMDataBank
Unified global portal for deposition and retrieval of 3DEM density maps, atomic models, and associated metadata
Resource for news, events, software tools, data standards, validation methods for the 3DEM community

4. Advisory Committee (current):
EMDBAC 30 September 2010
Project Team
Baylor College of Medicine
Wah Chiu, PI
Steven Ludtke
Corey Hryc
Grigore Pintilie
Matthew Baker
Matthew Dougherty
Rutgers University
Helen Berman, co-PI
Catherine Lawson
Raul Sala
Brian Hudson
John Westbrook
European Bioinformatics
Institute
Gerard Kleywegt, co-PI
Ardan Patwardhan
Eduardo Sanz Garcia
Ingvar Lagerstedt
Matthew Conroy
Advisory Committee (current):
Paul Adams (chair), Richard Henderson,
Bram Koster, Andrej Sali, Maryanne Martone

5. Project Goals
In Collaboration with the 3DEM community:
Establish data-validation methods that can be used during structure determination
Define key indicators of a well-determined 3DEM structure
Implement validation procedures for 3DEM maps and models in the deposition pipeline

6. Early Steps...See reprints in your folders
EM Validation Task Force
Henderson et al. (2012) “Standards for assessing Maps and Model fit into maps need to be developed.”
1st CryoEM Modelling Challenge
Biopolymers Special Issue
September 2012
3DEM Community-Supported Challenges
Particle Picking (Zhu et al 2004) ; CTF (Marabini et al 2015)

7. 3DEM Validation Report Development
Model-only validation (as recommended by X-ray TF) has been implemented for 3DEM:
Special concerns for 3DEM coordinate models:
Polymer Trace Conformation (Cα, P)
RNA conformation
Clashes (e.g., from rigid body fits)
3J6B: EM structure of yeast large ribosomal 3.2 Å
report implemented and under internal review.
we can include handouts of 3DEM validation reports in the folders for the advisors to review and comment.

8. Watch EMDataBank News for Details
Challengers and Assessors
FOR MAPS
create/evaluate single particle reconstructions from seven benchmark datasets 
FOR MODELS
create/evaluate coordinate models from moderate to high resolution 3DEM reconstructions 
Watch EMDataBank News for Details
Wanted
3DEM
GRC
POSTER

9. The Map Challenge Committee:
Bridget Carragher (Chair), Jose-Maria Carazo, Wen Jiang, John Rubinstein, Peter Rosenthal, Fei Sun, Janet Vonck
Targets:
Raw Datasets deposited at EMPIAR (PDBe)

10. The Map Challenge Anticipated Timing:
Registration opens late June 2015
Challengers: reconstruction submissions* open July through December 2015
Assessors: open data assessment period commences early 2016
Results Workshop mid-2016
*committee-developed questionnaire with file uploads

11. The Model Challenge Committee: Targets & Timing:
Paul Adams (Chair), Axel Brunger, Randy Read, Torsten Schwede, Maya Topf, Gerard Kleywegt
Targets & Timing:
To be determined at a committee workshop in June. Targets will be selected from recent maps in the 3-5 Ångstrom resolution range.
The Model Challenge

12. The Model Challenge
Possible targets:

13. Charge for Sunday