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Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays  Daniel T. Croft, Rick.

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Presentation on theme: "Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays  Daniel T. Croft, Rick."— Presentation transcript:

1 Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays  Daniel T. Croft, Rick M. Jordan, Heather L. Patney, Craig D. Shriver, Marina N. Vernalis, Trevor J. Orchard, Darrell L. Ellsworth  The Journal of Molecular Diagnostics  Volume 10, Issue 3, Pages (May 2008) DOI: /jmoldx Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 DNA extraction (A) and whole-genome amplification (B) yields from 200-μl aliquots of serum or plasma collected over a 20-year period from 12 individuals. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Genomic distribution of SNPs yielding discordant genotypes between wgaDNA derived from stored serum/plasma and gDNA isolated from peripheral blood on NspI (A) and StyI (B) arrays. The centromeres are indicated by gray bars. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Genomic distribution of SNPs showing homozygote conversion between wgaDNA derived from stored serum/plasma and gDNA isolated from peripheral blood on NspI (A) and StyI (B) arrays. The centromeres are indicated by gray bars. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions


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