1. Lab Safety & Microscope Use

2. Learning Goal 1. I will remind myself how to act safely in a lab
2. I will learn how to use and focus a compound and dissecting microscope
3. I will learn how to calculate the Field of View of a microscope
4. I will learn how to calculate the magnification of a specimen drawing

3. What is unsafe or out of the place in the below picture?
Discuss as a table team and be ready to report out

4. Compound microscope
When would you use it?
Dissection (stereoscope) microscope
When would you use it?

5. Biology 11/12 Space
How do we keep it clean?

6. Drawers will have labels and pictures
YOU will be responsible for finding items
YOU will be responsible for putting items back

7. Broken glass is to be swept up and put into container on Chemistry counter
Tell Mrs. Becker if anything breaks
Clean up immediately so that no one steps on glass
On any day we are in the lab, no open toed shoes for safety from broken glass

8. Fire Exit
Lab has two fire doors. Head outside and we will meet on the back field. FIND ME if we do not head out together
In the classroom, head out the door at the back of the classroom

9. Fire Blanket
If you catch your clothing on fire, there are 3 fire blankets in the lab
Wrap around yourself or your partner and STOP DROP and ROLL to suffocate the flame

10. Eye Wash Station
Anything in your eye, you need 20 minutes of flushing your eye out of water. Hold your eye open.
TELL MRS. BECKER right away.

12. Compound Microscope

13. Dissecting Microscope

14. Scanning Power Low Power High Power Oil Immersion
Exercise #1: Magnification
Scanning Power
Low Power
High Power
Oil Immersion

15. Exercise #2: Calculating Field of View
Ruler under scanning power
Filament of spirolina under scanning power

16. What happens at high and oil immersion?
Calculating Field of View
Scanning power
10 x ocular
4 x objective
total magnification = 40X
What happens at high and oil immersion?
Low power
10 x ocular
10 x objective
total magnification = 100X

17. Medium is low in our naming.
You will sometimes see “medium power”. We are not using that, (but be aware it is another naming method).
Medium is low in our naming.
Scanning
Low
High
Oil immersion

18. Changing Units To Micrometers
5 mm = ___________µm
4 cm = ____________µm
0.2 mm = __________µm

19. MUST BE IN THE SAME UNITS!
Drawing Magnification
Magnification = Drawing size
Actual size
MUST BE IN THE SAME UNITS!

20. When looking at pond water you see a paramecium that covers 1/6 of the FOV in low power. How big is the paramecium?

21. In Kade's lab report, he drew a diagram of a paramecium
In Kade's lab report, he drew a diagram of a paramecium. An actual paramecium is m. What is the magnification of Kade's diagram?
7.2 cm

22. Exercise #3: Prepare a Wet Mount

23. Exercise #5: Dissection Scope Diagram
Use any of the invertebrates from the tray in the lab and look and draw under the stereoscope. What is the total magnification of your image?

24. Today's To Do List: 1. Work through exercise #1 - 5 with your partner
2. Answer the questions at end of lab at home this evening