1. Volume 6, Issue 3, Pages 768-780 (May 2013)
Disruption of Secondary Wall Cellulose Biosynthesis Alters Cadmium Translocation and Tolerance in Rice Plants 
Xue-Qin Song, Li-Feng Liu, Yi-Jun Jiang, Bao-Cai Zhang, Ya-Ping Gao, Xiang-Ling Liu, Qing-Shan Lin, Hong-Qing Ling, Yi-Hua Zhou 
Molecular Plant 
Volume 6, Issue 3, Pages (May 2013)
DOI: /mp/sst025
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Characterization of the Phenotypes in Wild-Type and bc13 Mutant Plants.
(A, B) Three-month-old wild-type (A) and bc13 plants (B).
(C, D) Measurement of the force and extension length required for breaking wild-type and bc13 mutant internodes and leaves. Bar = 15 cm in (A) and (B).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Examination of the Secondary Cell-Wall Structure in Wild-Type and bc13 Mutant Plants.
(A–D) TEM micrographs of the cross-sections of sclerenchyma cells in wild-type and its high magnification (A, C) and those in bc13 and its magnification (B, D). Note a reduction in wall thickness in bc13. Bars = 5 μm in (A) and (B); and 2 μm in (C) and (D).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

4. Figure 3 Map-Based Cloning of the BC13 Gene.
AThe bc13 locus was mapped to two BAC clones on chromosome 9. Sequencing analysis revealed a point mutation that results one amino acid change at the 101th residue.
BA construct for complementary assay.
CA CAPS marker (digested by BseRI) was developed to distinguish the wild-type and bc13 background.
DFolding the internodes of rice plants (indicated by the arrows) to show the rescued mechanical property in the complemented plants.
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Examination of Cd Content in the Wild-Type and bc13 Seedlings after Cd Treatments.
AThe test seedlings were divided into three parts for Cd content assay.
BCd content in the continuous parts of the wild-type and bc13 seedlings, showing that bc13 has high Cd content in roots, but low Cd content in leaves. Data of mean ± SE (n = 3, * P < 0.05 and ** P < 0.01 by Student’s t-test). DW, dry weight.
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Expression Assay of the Genes Involved in Cd Uptake and Transport.
Exploring the relative expression levels of the targeting genes in roots, leaf sheathes, and leaves in the wild-type and bc13 seedlings treated and untreated with Cd. Data of mean ± SE (n = 3, * P < 0.05 and ** P < 0.01 by Student’s t-test).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
Cell-Wall Composition and the Cd Content in the Cell-Wall Residues of Wild-Type and bc13 Plants.
(A, B) Cell-wall composition of roots (A) and shoots (B).
(C, D) Cd retention in the cell-wall residues of roots (C) and shoots (D). Data of mean ± SE (n = 3, * P < 0.05 and ** P < 0.01 by Student’s t-test).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

8. Figure 7 Expression Profiles of CESA9/BC13.
(A–F) GUS activity assay of the BC13 promoter::GUS transgenic plants. GUS signals were showed in vascular bundles and sclerenchymatous cells in roots (A, B), internodes (C, a vascular bundle embedded at the left corner), and the coleoptiles (D–F), including metaxylem and metaphloem (B, F).
GRelative expression levels of BC13 in roots and leaves with or without Cd treatments.
HGUS activity was significantly quenched in roots and leaves (embedded figures) after Cd treatment. Mp, metaphloem; Mx, metaxylem; Sc, sclerenchyma cells; V, vascular bundle. Bar = 150 μm in (A), (C), and (E); 100 μm in (B) and (F); and 3 mm in (D). Data of mean ± SE (n = 3, **P < 0.01 by Student’s t-test).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

9. Figure 8
Comparison of Vascular Structure in the Internodes and Root Steles between Wild-Type and bc13.
(A–F) Cross-sections of the wild-type (A, C) and bc13 (B, D–F) internodes, showing that besides normal vascular bundles (B, D), bc13 also has abnormal ones as indicated by red arrows (E, F).
(G–J) Cross-sections of the wild-type (G) and bc13 (H–J) root steles, showing that bc13 has collapsed xylem and abnormal metaxylem as indicated by red arrows (I, J). Cmx, central metaxylem; Mp, metaphloem; Mx, metaxylem. Bar = 350 μm in (A) and (B); 200 μm in (C)–(F); and 50 μm in (G)–(J).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

10. Figure 9
Examination of the Cd Concentration in the Xylem Sap of Wild-Type and bc13 Plants under Different Cd Treatments.
Seedlings were exposed to the uptake solution containing 10 μM and 25 μM CdCl2 for 12 h. Data of mean ± SE (n = 3, * P < 0.05 and ** P < 0.01 by Student’s t-test).
Molecular Plant 2013 6, DOI: ( /mp/sst025)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions