1. Houston et al., 2009 Figure S1 A B
4 WAF
3 WAF
5 WAF
6 WAF
3.0
2 WAF pI kD
205
116 97 84 66 55 45 36 29 24 20
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Figure S1. Two dimensional reference maps of castor proteins isolated from seeds of 2, 3, 4, 5, and 6 WAF. Total protein was isolated from the five experimental stages of castor seed. Protein extracts (0.05 mg) were labeled with Cy5, separated by wide and narrow IPG strips, and fractionated by 2-DGE. A, Protein extracts were separated with pH 3-10, wide-range IPG strips. B, Proteins were fractionated using narrow-range IPG strips, pH range 4 to 7. Migrating positions of isoelectric point (pI) and molecular mass (kD) markers are at the top and left of the gels, respectively.