Presentation is loading. Please wait.

Presentation is loading. Please wait.

An Alternatively Spliced IL-15 Isoform Modulates Abrasion-Induced Keratinocyte Activation  Tsung-Lin Lee, Mei-Ling Chang, Yu-Jei Lin, Ming-Hsun Tsai,

Published byLeony Budiono Modified over 5 years ago

Similar presentations

Presentation on theme: "An Alternatively Spliced IL-15 Isoform Modulates Abrasion-Induced Keratinocyte Activation  Tsung-Lin Lee, Mei-Ling Chang, Yu-Jei Lin, Ming-Hsun Tsai,"— Presentation transcript:

1 An Alternatively Spliced IL-15 Isoform Modulates Abrasion-Induced Keratinocyte Activation 
Tsung-Lin Lee, Mei-Ling Chang, Yu-Jei Lin, Ming-Hsun Tsai, Yi-Hsuan Chang, Che-Ming Chuang, Yun Chien, Tomasz Sosinowski, Chih-Hsiu Wang, Yi-Yuan Chen, Chien-Kuo Lee, Jau-Shiuh Chen, Li-Fang Wang, John T. Kung, Chia-Chi Ku  Journal of Investigative Dermatology  Volume 135, Issue 5, Pages (May 2015) DOI: /jid Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 An N-ethyl-N-nitrosourea-induced point mutation in exon 7 of the Il15gene results in prevalent expression of IL-15ΔE7 isoform in deficient memory (DM) mouse skin. (a) IL-15 reverse transcriptase–PCR analysis of wild type (lane 1) and DM (lane 2) peripheral blood leukocytes by Caliper LabChip GX, 1 K chip (Perkin Elmer, Waltham, MA). (b) IL-15 reverse transcriptase–PCR analysis of 5000 sorted B6 (lanes 1, 3, 5, and 7) and DM (lanes 2, 4, 6, and 8) spleen, macrophages (lanes 1 and 2), dendritic cells (lanes 3 and 4), T cells (lanes 5 and 6), and B cells (lanes 7 and 8). (c) One of two reverse transcriptase–PCR products for P191 (DM) was identical with the B6 DNA sequence, and the other with complete missing of the 5′ end of exon 7. (d) Measurement (mean±SEM) of expression of IL-15ΔE7, IL-15, and total IL-15 messenger RNA in wild type (n=4), DM (n=8), or Il15+/- (n=4) epidermis by real-time PCR (unpaired Student’s t-test). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Abrasion-induced skin thickening, keratinocyte proliferation, and neutrophil infiltration are less in deficient memory (DM) skin. (a) Paraffin skin sections of wild type, DM, and Il15-/- mice were stained by hematoxylin and eosin for histological analysis. E, epidermis; D, dermis. Bars=100 μm. (b) A 1-mm segment from hematoxylin and eosin-stained sections from either normal (n=4) or abraded (n=4–5) skin was selected for quantitative analysis (mean±SEM, unpaired Student’s t-test; n.s., not significant). (c–f) Normal and abraded (48 hours) skin sections were stained with anti-Ki67 (c) or anti-Ly6G (e) antibody for immunohistochemical analysis. Brown=DAB; bar=50 μm (mean±SEM, n=4–5 each group; two-way analysis of variance followed by Bonferroni’s post hoc test). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Abrasion-induced neutrophil infiltration is reduced in deficient memory (DM) skin graft on wild-type host. (a) Photographs of skin grafts on the recipients as indicated at 7, 30, and 40 days after transplantation. (b) Skin grafts at day 40 were abraded, excised at 48 hours and the sections were stained for hematoxylin and eosin analysis. Bars=100 μm in large and 20 μm in small panels. (c) Graphs represent enumeration of neutrophils underneath the injured epidermis by hematoxylin and eosin analysis. Results shown are one representative experiment from two independent experiments. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Ectopic expression of IL-15ΔE7 in the epidermis profoundly inhibits keratinocyte proliferation, activation, and neutrophil infiltration into abraded skin. Expression of pIL-15 or pIL-15ΔE7 in wild-type skin was analyzed by (a) quantitative real-time reverse-transcriptase–PCR and (b) ELISA (n=2, each time point). Abraded skin sections at 24 hours were stained with hematoxylin and eosin (c) or with anti-Ki67 (d) or anti-Ly6G (e) antibody for immunohistochemical analysis (mean±SEM, n=5 for unabraded skin; n=3–4 for abraded skin. Two-way analysis of variance followed by Bonferroni’s post hoc test was performed. (f) Time-course quantitative real-time reverse-transcriptase–PCR analysis of the Sox9 or loricrin gene was expressed by relative expression compared with β-actin in each sample. (g) Production of keratinocyte and G-CSF from skin tissues (0.5 × 0.5 cm2) was assayed by ELISA. Each circle represents one tissue. Data shown are representative from three independent experiments. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Overexpression of IL-15ΔE7 inhibits neutrophil infiltration to SDS and IMQ-treated skin. Wild type, deficient memory (DM), and IL-15ΔE7-expressing wild-type mice were topically treated with 10% SDS for 24 hours (a, b) or with IMQ (c, d) for 3 or 6 consecutive days. Skin sections were stained for hematoxylin and eosin analysis or with anti-Ly6G antibody for immunohistochemical analysis. The number of Ly6G+ cells was enumerated (mean±SEM, n=3 for SDS treatment; n=4 for IMQ treatment. Two-way analysis of variance followed by Bonferroni’s post hoc test; n.d., not detectable). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 IL-15ΔE7 suppresses phosphorylation of STAT-5 and expression of bcl-2 mRNA in HT-2 cells. HT-2 cells (1 × 106) were starved for 4 hours and stimulated with recombinant GST fusion IL-15 (IL-15/GST) or IL-15ΔE7 (IL-15ΔE7/GST) protein (400 ng ml-1), or left untreated for 2 hours. (a) Protein lysate (25 μg) was subjected to 7.5% SDS-PAGE and probed with rabbit polyclonal anti-phosphoSTAT5, anti-STAT-5, or anti-α tubulin antibody for immunoblot analysis. (b) Expression of bcl-2 messenger RNA was measured by quantitative real-time reverse transcriptase–PCR and normalized to β-actin messenger RNA. Results are expressed by fold change compared with untreated control. Each bar represents the mean±SEM from technical duplicates of two independent measurements (one-way analysis of variance, Tukey’s multiple comparison test). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "An Alternatively Spliced IL-15 Isoform Modulates Abrasion-Induced Keratinocyte Activation  Tsung-Lin Lee, Mei-Ling Chang, Yu-Jei Lin, Ming-Hsun Tsai,"

Similar presentations

The Suppressor of Cytokine Signaling-3 Is Upregulated in Impaired Skin Repair: Implications for Keratinocyte Proliferation  Itamar Goren, Andreas Linke,

The Suppressor of Cytokine Signaling-3 Is Upregulated in Impaired Skin Repair: Implications for Keratinocyte Proliferation  Itamar Goren, Andreas Linke,
The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis  Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,

The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis  Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,
Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.
Cdc42 Inhibits ERK-Mediated Collagenase-1 (MMP-1) Expression in Collagen-Activated Human Keratinocytes  Maryam G. Rohani, Brian K. Pilcher, Peter Chen,

Cdc42 Inhibits ERK-Mediated Collagenase-1 (MMP-1) Expression in Collagen-Activated Human Keratinocytes  Maryam G. Rohani, Brian K. Pilcher, Peter Chen,
Syk Mediates IL−17-Induced CCL20 Expression by Targeting Act1-Dependent K63- Linked Ubiquitination of TRAF6  Nan-Lin Wu, Duen-Yi Huang, Hsin-Ni Tsou, Ying-Cing.

Syk Mediates IL−17-Induced CCL20 Expression by Targeting Act1-Dependent K63- Linked Ubiquitination of TRAF6  Nan-Lin Wu, Duen-Yi Huang, Hsin-Ni Tsou, Ying-Cing.
Gene Expression Profiling of the Leading Edge of Cutaneous Squamous Cell Carcinoma: IL-24-Driven MMP-7  Hiroshi Mitsui, Mayte Suárez-Fariñas, Nicholas.

Gene Expression Profiling of the Leading Edge of Cutaneous Squamous Cell Carcinoma: IL-24-Driven MMP-7  Hiroshi Mitsui, Mayte Suárez-Fariñas, Nicholas.
Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1  Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,

Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1  Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,
An Alternatively Spliced IL-15 Isoform Modulates Abrasion-Induced Keratinocyte Activation  Tsung-Lin Lee, Mei-Ling Chang, Yu-Jei Lin, Ming-Hsun Tsai,

An Alternatively Spliced IL-15 Isoform Modulates Abrasion-Induced Keratinocyte Activation  Tsung-Lin Lee, Mei-Ling Chang, Yu-Jei Lin, Ming-Hsun Tsai,
Fas/Fas Ligand Mediates Keratinocyte Death in Sunitinib-Induced Hand-Foot Skin Reaction  Chun-Nan Yeh, Wen-Hung Chung, Shih-Chi Su, Yen-Yang Chen, Chi-Tung.

Fas/Fas Ligand Mediates Keratinocyte Death in Sunitinib-Induced Hand-Foot Skin Reaction  Chun-Nan Yeh, Wen-Hung Chung, Shih-Chi Su, Yen-Yang Chen, Chi-Tung.
Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes  Zheng Jun Li, Dae Kyoung Choi, Kyung Cheol Sohn, Min Seok Seo, Hae Eul Lee,

Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes  Zheng Jun Li, Dae Kyoung Choi, Kyung Cheol Sohn, Min Seok Seo, Hae Eul Lee,
Topical ROR Inverse Agonists Suppress Inflammation in Mouse Models of Atopic Dermatitis and Acute Irritant Dermatitis  Jun Dai, Min-Kyung Choo, Jin Mo.

Topical ROR Inverse Agonists Suppress Inflammation in Mouse Models of Atopic Dermatitis and Acute Irritant Dermatitis  Jun Dai, Min-Kyung Choo, Jin Mo.
Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation 

Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation 
Enhanced Inflammation and Accelerated Wound Closure Following Tetraphorbol Ester Application or Full-Thickness Wounding in Mice Lacking Hyaluronan Synthases.

Enhanced Inflammation and Accelerated Wound Closure Following Tetraphorbol Ester Application or Full-Thickness Wounding in Mice Lacking Hyaluronan Synthases.
Decreased Expression of Caveolin-1 Contributes to the Pathogenesis of Psoriasiform Dermatitis in Mice  Yukie Yamaguchi, Yuko Watanabe, Tomoya Watanabe,

Decreased Expression of Caveolin-1 Contributes to the Pathogenesis of Psoriasiform Dermatitis in Mice  Yukie Yamaguchi, Yuko Watanabe, Tomoya Watanabe,
Yasuyo Sano, Jin Mo Park  Journal of Investigative Dermatology 

Yasuyo Sano, Jin Mo Park  Journal of Investigative Dermatology 
Volume 15, Issue 2, Pages (February 2009)

Volume 15, Issue 2, Pages (February 2009)
CYLD Inhibits Melanoma Growth and Progression through Suppression of the JNK/AP-1 and β1-Integrin Signaling Pathways  Hengning Ke, Christina K. Augustine,

CYLD Inhibits Melanoma Growth and Progression through Suppression of the JNK/AP-1 and β1-Integrin Signaling Pathways  Hengning Ke, Christina K. Augustine,
Stromal Fibroblast–Specific Expression of ADAM-9 Modulates Proliferation and Apoptosis in Melanoma Cells In Vitro and In Vivo  Anna N. Abety, Jay W. Fox,

Stromal Fibroblast–Specific Expression of ADAM-9 Modulates Proliferation and Apoptosis in Melanoma Cells In Vitro and In Vivo  Anna N. Abety, Jay W. Fox,
Tumor Necrosis Factor-α-Activated Human Adipose Tissue–Derived Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing through Paracrine Mechanisms 

Tumor Necrosis Factor-α-Activated Human Adipose Tissue–Derived Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing through Paracrine Mechanisms 
Preclinical Studies of a Specific PPARγ Modulator in the Control of Skin Inflammation  Arianna Mastrofrancesco, Daniela Kovacs, Massimiliano Sarra, Emanuela.

Preclinical Studies of a Specific PPARγ Modulator in the Control of Skin Inflammation  Arianna Mastrofrancesco, Daniela Kovacs, Massimiliano Sarra, Emanuela.

Similar presentations

Ads by Google