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Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L

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1 Suppression of the immunologic response to peanut during immunotherapy is often transient 
Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L. Guerrerio, MD, PhD, Kristin L. Chichester, MS, Corinne A. Keet, MD, MD, Anja P. Bieneman, BS, Robert G. Hamilton, PhD, Robert A. Wood, MD, John T. Schroeder, PhD, Pamela A. Frischmeyer-Guerrerio, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 135, Issue 5, Pages (May 2015) DOI: /j.jaci Copyright © Terms and Conditions

2 Fig 1 SHR and constitutive expression of basophil activation markers during immunotherapy. SHR (A), constitutive CD63 expression (B), and CD203c mean fluorescence intensity (MFI; C) were measured in basophil-enriched suspensions from subjects undergoing OIT (left panels) and SLIT (right panels) for peanut. T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

3 Fig 2 Effect of OIT and SLIT on basophil CD63 expression and HR in response to peanut. Basophil-enriched suspensions were stimulated with crude peanut extract at 0.1 ng/mL (A), 1 ng/mL (B), and 10 ng/mL (C). SHR and CD63 expression in medium alone were subtracted to obtain stimulated values. T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

4 Fig 3 Changes in basophil IL-4 expression during peanut OIT and SLIT. Basophil expression of IL-4 was measured by using intracellular flow cytometry after incubation of whole-blood samples with peanut (A), anti-IgE (B), dust mite at 1 AU/mL (C) or 10 AU/mL (D), and ionomycin (E). T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

5 Fig 4 Effect of peanut OIT and SLIT on TH2 cytokine responses in DC–T-cell cocultures. IL-5 and IL-13 were measured in supernatants from cocultures of pDCs and mDCs with autologous CD4+ T cells stimulated with 50 μg/mL crude peanut extract (A) or 100 AU/mL dust mite (B). Spontaneous cytokine secretion, as measured in medium alone, was subtracted to obtain allergen-induced values. T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

6 Fig 5 Effect of peanut OIT and SLIT on DC expression of costimulatory molecules and HLA-DR. Mean fluorescence intensity (MFI) of CD40, CD86, and HLA-DR on pDCs (left 2 columns) or mDCs (right 2 columns) was measured after coculture with CD4+ T cells stimulated with 50 μg/mL peanut (A) or 100 AU/mL dust mite (B). T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

7 Fig 6 Effect of peanut OIT and SLIT on expression of CD80 on mDCs. CD80 mean fluorescence intensity (MFI) was measured on mDCs after coculture with CD4+ T cells stimulated with media alone (A), 50 μg/mL peanut (B), or 100 AU/mL dust mite (C). T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

8 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

9 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

10 Fig E3 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

11 Fig E4 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

12 Fig E5 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

13 Fig E6 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

14 Fig E7 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

15 Fig E8 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

16 Fig E9 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

17 Fig E10 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

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