1. CLB5-Dependent Activation of Late Replication Origins in S. cerevisiae
Anne D Donaldson, M.K Raghuraman, Katherine L Friedman, Frederick R Cross, Bonita J Brewer, Walton L Fangman 
Molecular Cell 
Volume 2, Issue 2, Pages (August 1998)
DOI: /S (00)

2. Figure 1 2D Gel Analysis of Origin Use in CLB and clb5 Strains
CLB (top panels) and clb5 (bottom panels). The names of the three early and four late origins examined are above the panels. Origin activation times during a normal S phase are expressed as replication indices (R.I.; see text).
Molecular Cell 1998 2, DOI: ( /S (00) )

3. Figure 2
Partial Suppression of the clb5 Late Origin Activation Defect in a clb5 clb6 Strain
DNA was prepared from asynchronously growing CLB, clb6, clb5, and clb5 clb6 cultures. 2D gels were run and probed to examine the early origin ARS607, and late origins ARS501 and ARS603. For CLB and clb5 strains, the gels are those shown in Figure 1, repeated here for ease of comparison.
Molecular Cell 1998 2, DOI: ( /S (00) )

4. Figure 3
Effect of clb Mutations on Direction of Replication Fork Movement Close to Late Origin ARS603
(A) Chromosome map of the sequences to the right of late origin ARS603. XbaI fragments were digested in-gel with PstI, and blots were probed for the larger XbaI-PstI fragment produced. If ARS603 fires, a replication fork will move through the XbaI fragment to the right (solid arrow). Under conditions where ARS603 is inactive, the fragment would be replicated by a leftward-moving fork (dashed arrow). The chromosome map is not drawn to scale.
(B) The Y arc resulting from rightward replication fork movement through the XbaI fragment is shown by a solid arc. The dashed arc illustrates the Y arc observed if the replication fork movement is leftward.
(C–F) Autoradiographs show fork direction gels run on DNA prepared from asynchronously growing CLB, clb6, clb5, and clb5 clb6 strains.
Molecular Cell 1998 2, DOI: ( /S (00) )

5. Figure 4
Efficiency of Early and Late Origin Activation in clb Mutant Strains
Activation efficiencies were estimated for two early origins (ARS607 and ARS305) and two late origins (ARS603 and ARS501) from fork direction analyses such as those shown in Figure 3. See Experimental Procedures for further details.
Molecular Cell 1998 2, DOI: ( /S (00) )

6. Figure 5
Kinetics of DNA Replication in CLB, clb5, and clb5 clb6 Mutant Strains after Release from α Factor Arrest
Dotted lines in each panel indicate the budding time (Tbud), defined as the time at which the percentage of cells with visible buds was at half its final level. Release from α factor arrest was slightly accelerated in the clb mutant strains, as determined by the kinetics of budding. This effect was reproducible but has not been observed in previous studies of clb mutants. The faster release here may result from uracil and leucine prototrophies in the clb mutants. The clb mutant strains were clb5::URA3 leu2 and clb5::URA3 clb6::LEU2, whereas the CLB strain was ura3 leu2.
Molecular Cell 1998 2, DOI: ( /S (00) )

7. Figure 6
Replication Times of Seven Sequences Compared to the Time of Bud Emergence in CLB, clb5, and clb5 clb6 Strains
The replication time (Trep) for a given fragment is defined as the time at which half of the final level of replication has occurred. Replication times of seven sequences were obtained from the experiments shown in Figure 5 and are listed in Table 1. Here the replication times are plotted relative to time of budding (Tbud, assigned to be 0 min in each of the three experiments for this plot).
Molecular Cell 1998 2, DOI: ( /S (00) )

8. Figure 7
Model of the Ability of B-Type Cyclins to Promote S Phase and Activate Replication Origins
(A), (B), and (C) show time lines (not to scale) representing the cell cycle in CLB, clb5, and clb5 clb6 strains, respectively. Arrows indicate the time of bud emergence during the cell cycle, and the duration of S phase in each case is drawn as an open box. Firing of the various replication origins is shown by shaded circles. Light shading represents origins activated early in S phase, and darker shading those origins activated later. Hatched bars represent the proposed capability of different Clb-Cdk1p activities to promote timely firing of early and late replication origins. See text for further discussion.
Molecular Cell 1998 2, DOI: ( /S (00) )