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Specific isoforms of leucine-rich α2-glycoprotein detected in the proliferative endometrium of women undergoing assisted reproduction are associated with.

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Presentation on theme: "Specific isoforms of leucine-rich α2-glycoprotein detected in the proliferative endometrium of women undergoing assisted reproduction are associated with."— Presentation transcript:

1 Specific isoforms of leucine-rich α2-glycoprotein detected in the proliferative endometrium of women undergoing assisted reproduction are associated with spontaneous pregnancy  David J. Gillott, Ph.D., Hazem M. Al-Rumaih, M.D., Kit-Yi Leung, Ph.D., Abdallah Eldib, M.Sc., J. Gedis Grudzinskas, M.D.  Fertility and Sterility  Volume 90, Issue 3, Pages (September 2008) DOI: /j.fertnstert Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Two-dimensional gel of uterine flushing showing the location of the train of leucine-rich α2-glycoprotein isoforms. Protein spots were identified by excision and in-gel trypsin digestion followed by high-performance liquid chromatography and tandem mass spectrometry. Identified amino acid sequences were used to search the protein sequence database maintained by the Swiss Institute of Bioinformatics (ExPASy, Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Graphical representation of normalized mean spot volumes (y-axis) in pregnant and nonpregnant women (± standard error). (A) Analyzed by diagnostic group and according to spontaneous pregnancy, treatment-independent pregnancy, and nonpregnant. (B) Detail of two-dimensional gel showing position of relevant leucine-rich α2-glycoprotein isoforms. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Graphic representation of expression of (A) LRGiii and (B) LRGiv (bottom) for the spontaneous (spon), treatment-related pregnancies (treat), and nonpregnant groups for the diagnoses of male factor infertility, tubal disease, polycystic ovary syndrome, and unexplained infertility. The y-axis represents normalized volume. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Example of an ms/ms spectrum, in this case for the peptide ENQLEVLEVSWLHGLK (amino acid 149–164). The x-axis represents mass/charge (m/z). The spikes labeled y3–y13 represent the detection of variously sized peptide fragments. The individual amino acids are superimposed over the detected fragments showing the amino acid sequence of the fragmented peptide. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions


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