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Comparative Evaluation of Four Real-Time PCR Methods for the Quantitative Detection of Epstein-Barr Virus from Whole Blood Specimens  Daelynn Buelow,

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Presentation on theme: "Comparative Evaluation of Four Real-Time PCR Methods for the Quantitative Detection of Epstein-Barr Virus from Whole Blood Specimens  Daelynn Buelow,"— Presentation transcript:

1 Comparative Evaluation of Four Real-Time PCR Methods for the Quantitative Detection of Epstein-Barr Virus from Whole Blood Specimens  Daelynn Buelow, Yilun Sun, Li Tang, Zhengming Gu, Stanley Pounds, Randall Hayden  The Journal of Molecular Diagnostics  Volume 18, Issue 4, Pages (July 2016) DOI: /j.jmoldx Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Linearity, limits of detection, and limits of quantification for type 1 by each method. Linearity of artificially seeded whole blood samples with type 1 as measured by real-time PCR. A total of nine replicates of each concentration were tested, and all replicates were plotted against concentrations determined by droplet digital PCR. Ct, cycle threshold; LDT, laboratory-developed test. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Colinear regression of type 1 and type 2 in artificially seeded whole blood samples. Epstein-Barr virus type 1 and type 2 were both serial diluted, extracted, and used to generate the linear regression lines. Ct was plotted against the droplet digital PCR concentrations. Graphs represent the three analyte-specific reagents (A–C) and one LDT (D) evaluated. Ct, cycle threshold; LDT, laboratory-developed test. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Quantitative pairwise correlation of patient samples between the different methods. Values are represented in log10 copy/mL. Intercept, slope, and R2 for each comparison are indicated within each graph, with numbers in parentheses indicating the 95% CI. Comparison between Luminex and LDT (A), Luminex and Focus (B), Luminex and ELITech (C), ELITech and LDT (D), ELITech and Focus (E), and Focus and LDT (F). LDT, laboratory-developed test. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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