1. OmpA C-Terminus Library
5uL of ligation reaction (4500 !! ng DNA) were transformed into chemically competent BL29 Gold DE3 Cells.
5uL
50uL
200uL
Vector 2 33
lots
10mer 14
149
15mer 6
275
uL transformant vs. colony count

2. Biobricking the Fec System
Construct Features:
Swappable FecA - FecA is flanked by Nhe1 and AflII sites to allow the easy mutagenesis and replacement of FecA.
Variable Promoters - each component will be on a separate constitutive promoter.
The optimization of GFP expression using promoters of different strengths is planned.

3. Biobricking the Fec System
Mutagenesis of Fec promoter to weaken gene expression, providing a range of sensitivity.
Mutagenesis of the Fec promoter to remove FUR repressor binding site, allowing easier assays.
Studies detailing the response of reporter expression to mutations at each site of the promoter have been done. (Enz, Pressler, Braun papers)