1. Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro 
E. Tognana, Ph.D., F. Chen, M.D., R.F. Padera, M.D., Ph.D., H.A. Leddy, M.S., S.E. Christensen, B.S., F. Guilak, Ph.D., G. Vunjak- Novakovic, Ph.D., L.E. Freed, M.D., Ph.D. 
Osteoarthritis and Cartilage 
Volume 13, Issue 2, Pages (February 2005)
DOI: /j.joca
Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

2. Fig. 1
Integration at the 100μm scale. Histological appearance of constructs cultured as composites with rings of (A–D) articular cartilage, (E–H) devitalized bone or (I–L) vital bone for (A,B,E,F,I,J) 4 weeks or (C,D,G,H,K,L) 8 weeks in vitro. Representative sections were stained with safranin-O/fast green. Scale bars are: (A,C,E,G,I,K) 1.0mm and (B,D,F,H,J,L) 200μm.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

3. Fig. 2
Integration at the 10nm scale. Ultrastructural appearance of constructs cultured as composites with rings of (A,B,D) articular cartilage or (C) vital bone for 8 weeks in vitro. (A,C) Regions at the interface; (B,D) regions within construct away from the interface. Transmission electron micrography. Scale bars: (A–C) 5μm; (D) 200nm; Asterix indicates the scaffold. Arrow points towards the construct in a region close to the interface between the construct and the adjacent tissue.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

4. Fig. 3
Construct biomechanical properties. (A) Adhesive strength (kPa) and (B) compressive modulus (kPa) are shown after composite culture of engineered constructs with articular cartilage, devitalized bone or vital bone (AC, DB, or VB) for 3 days, 4 weeks, or 8 weeks (3d, 4w, 8w). Data represent the average±standard deviation of n=6 independent samples. Individual and interactive effects of culture duration and type of adjacent tissue (time, ring, time&ring) are indicated by P-value and post-hoc comparison. aSignificantly different from corresponding 4w sample. bSignificantly different from corresponding AC sample. cSignificantly different from corresponding VB sample.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

5. Fig. 4
Construct biochemical compositions. Amounts of (A,B) glycosaminoglycans (GAG) and total collagen, expressed as a percentages of construct wet weight (% wet wt), and (C) cells (per construct) are shown after composite culture of engineered constructs with articular cartilage, devitalized bone or vital bone (AC, DB, or VB) for 3 days, 4 weeks, or 8 weeks (3d, 4w, 8w). Data represent the average±standard deviation of n=6 independent samples. Individual and interactive effects of culture duration and type of adjacent tissue (time, ring, time&ring) are indicated by P-value and post-hoc comparison. aSignificantly different from corresponding 4w sample. bSignificantly different from corresponding AC sample. cSignificantly different from corresponding VB sample. NS=not significant.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

6. Fig. 5
Diffusivities of adjacent tissues. (A) Representative FRAP data for 70kDa dextran in articular cartilage showing bleached area and fluorescence recovery in images recorded at 0, 3, 8, and 18s (0s, 3s, 8s, 18s). Scale bar: 100μm. (B) Ratio of fluorescence recovery as a function of frequency-scaled time for 3kDa dextran in representative samples of articular cartilage (AC) and devitalized bone (DB), where q is the radius in the frequency domain. Lines show non-linear curve fits used to extract diffusion coefficients. (C) Diffusivities of 3 and 70kDa dextrans (open and closed bars, respectively) in AC and DB. Data are the average±standard deviation of n=6 independent samples. aSignificantly different from the corresponding 3kDa dextran.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions