1. Cytokine Expression Pattern in Bone Marrow Microenvironment after Allogeneic Stem Cell Transplantation in Primary Myelofibrosis 
Kais Hussein, Angelika Stucki-Koch, Haefaa Alchalby, Ioanna Triviai, Nicolaus Kröger, Hans Kreipe 
Biology of Blood and Marrow Transplantation 
Volume 22, Issue 4, Pages (April 2016)
DOI: /j.bbmt
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

2. Figure 1
Aberrant gene expression in PMF before and after transplantation. Mean and standard deviation are depicted by bars. Tx indicates treatment with ASCT. ∗P < .05,∗∗P < .01.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

3. Figure 2
Histological changes in PMF bone marrow before and after transplantation. (A) In this patient (case number 10; Supplementary Table 1), pre-ASCT bone marrow biopsy shows advanced stage PMF with aberrant megakaryocytic proliferation (arrows: enlarged and clustered cells; Giemsa, original magnifications ×200). (B) CD42b-staining highlights not only PMF megakaryocytes but also, as described earlier [21,33], the typical increase of interstitial proplatelet deposition (×400). (C) THBS1 is strongly expressed by aberrantly clustered megakaryocytes and is found in small particles, which represent megakaryocytic proplatelet depositions (×200). (D) Fibrosis with reticulin and collagen fibers and osteosclerosis represent the PMF cell–induced stromal changes in the bone marrow microenvironment (Gomorii silver, ×200). (E) After ASCT, the rebiopsy of the patient revealed hyperplastic bone marrow (hematoxylin and eosin, (×400). (F) CD42b reveals that megakaryocytes, which are donor-derived, show no aberrant clustering or increased proplatelet deposition. (G) Megakaryocytes express THBS1 but the number of megakaryocytes is almost normal. (H) In comparison to the PMF stage, after ASCT, the extracellular matrix is mainly normal and only focal areas with increased fibers indicate ongoing remodeling (Gomorii silver, ×200). Expressions of TIMPs are depicted in Supplementary Figure 1.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions