1. Effect of apelin on glomerular hemodynamic function in the rat kidney
Annette Hus-Citharel, Nadine Bouby, Alain Frugière, Laurence Bodineau, Jean-Marie Gasc, Catherine Llorens-Cortes 
Kidney International 
Volume 74, Issue 4, Pages (August 2008)
DOI: /ki
Copyright © 2008 International Society of Nephrology Terms and Conditions

2. Figure 1
Expression of relative apelin receptor mRNA in the four renal zones. Each bar represents the mean data±s.e.m., n=5 rats. aStatistically different from cortex, 0.025>P>0.01 (ANOVA).
Kidney International  , DOI: ( /ki )
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3. Figure 2
Expression of relative apelin receptor mRNA along the nephron. Results were expressed as the relative value from 2mm tubular length in percentage of the value from two Glom microdissected in the same run. Each bar represents the mean data±s.e.m. of 4–5 experiments.
Kidney International  , DOI: ( /ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

4. Figure 3
Detection by in situ hybridization of apelin receptor mRNA expression in glomerular arteriole. (a) Hybridization signal was observed in VEC (arrow) and in the VSMC (arrowheads) of arteriole (Art). A tangential section parallel to the axis of the arteriole showed labeling where the section cut only the endothelial cells (Figure 3a). (b) A nearly adjacent section hybridization with the sense probe showed no labeling but a faint background. Scale bar: 20μm.
Kidney International  , DOI: ( /ki )
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5. Figure 4
Effects of increasing doses of apelin 17 on diuresis rate in lactating female rats. Diuresis rate was measured in lactating female rats during two periods of 120min. Basal control period (hatched column) corresponds to intravenous injection of saline. Experimental period (closed column) corresponds to intravenous injection of increasing doses of apelin 17: 0.48, 1.90, and 3.80nmol per animal. Each column represents the mean data±s.e.m. expressed in μl/min per100g BW. *P<0.05 and **P<0.001 when compared to basal control period.
Kidney International  , DOI: ( /ki )
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6. Figure 5
Effect of apelin 17 on the juxtamedullary afferent arteriole contractile response to AngII. Arteriolar diameters were measured in the basal conditions, then 1min after adding 10−9M AngII and 1min after addition of 5 × 10−7M apelin 17 on vasoconstricted arterioles (n=8); **P<0.01.
Kidney International  , DOI: ( /ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

7. Figure 6
Effects of apelin 17 on the [Ca2+]i responses to AngII in juxtamedullary afferent arterioles. (a) A representative [Ca2+]i recording illustrating the [Ca2+]i drops observed when 5 × 10−7M apelin 17 was added during the plateau phase of 10−9M AngII-induced [Ca2+]i increase and when 10−8M BK was added during the plateau phase of a second 10−9M AngII-induced [Ca2+]i response is shown. (b) The time course of two successive applications of 5 × 10−7M apelin 17 followed by one application of 10−8M BK on the plateau phase of three different 10−9M AngII-induced [Ca2+]i responses is illustrated. (c) A representative recording illustrating the inhibition of the [Ca2+]i drop induced by 5 × 10−7M apelin 17 on the plateau phase elicited by 10−9M AngII when 2 × 10−5M L-NAME were superfused 10min before and during the AngII-induced [Ca2+]i response is shown. (d) Quantification of [Ca2+]i drop induced by apelin 17 in the absence or presence of L-NAME is shown. Each column represents the mean data±s.e.m. expressed in % of [Ca2+]i drop induced by apelin 17 in the presence (hatched column, n=14) or in the absence of L-NAME (closed column, n=6); **P<
Kidney International  , DOI: ( /ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

8. Figure 7
Effects of apelin 17 on [Ca2+]i responses to AngII in juxtamedullary muscular efferent arterioles. (a) A representative [Ca2+]i recording illustrating the [Ca2+]i drops produced when 5 × 10−7M apelin 17 was added during the plateau phase of the [Ca2+]i response induced by 10−9M AngII and when 10−8M BK was added during the plateau phase of the [Ca2+]i response induced by a second application of 10−9M AngII. (b) A representative [Ca2+]i recording illustrating the [Ca2+]i drops observed when 5 × 10−7M apelin 17 was added during the plateau phase of the [Ca2+]i responses induced by two successive applications of 10−9M AngII in the absence or presence of 2 × 10−5M L-NAME. L-NAME was superfused 10min before and during the second application of AngII. (c) Summarized data obtained in the absence and presence of L-NAME. Each column represents the mean data±s.e.m. expressed in % of [Ca2+]i drop induced by apelin 17 in the presence (hatched column, n=10) or in the absence (closed column, n=6) of L-NAME; **P<0.01.
Kidney International  , DOI: ( /ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

9. Figure 8
Effects of arteriolar de-endothelialization on the apelin 17-induced [Ca2+]i drop in juxtamedullary afferent arterioles. (a) A representative [Ca2+]i recording type performed on afferent arteriole with functional endothelium illustrating the [Ca2+]i drop observed when 5 × 10−7M apelin 17 was added during the plateau phase of 10−9M AngII-induced [Ca2+]i increase followed by an application of 10−8M BK. (b) showed a representative [Ca2+]i recording type performed on afferent arterioles without functional endothelium illustrating the inhibition of [Ca2+]i drop observed when 5 × 10−7M apelin 17 was added during the plateau phase of 10−9M AngII-induced [Ca2+]i increase followed by an application of 10−7M BK, used in so far as endothelium marker. The inset shows a representative [Ca2+]i recording illustrating the [Ca2+]i time course observed when 5 × 10−7M apelin 17 was added during the plateau phase of 10−9M AngII-induced [Ca2+]i increase in afferent arteriole without functional endothelium. (c) Summarized data obtained in the presence or absence of functional endothelium. Each column represents the mean data±s.e.m. expressed in % of [Ca2+]i drop induced by apelin 17 in the presence (closed column, n=14) or in the absence (hatched column, n=6) of functional endothelium; **P<0.001.
Kidney International  , DOI: ( /ki )
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10. Figure 9
[Ca2+]i responses induced by 5 × 10−7M apelin 17 in juxtamedullary afferent and muscular efferent arterioles. Δ[Ca2+]i is the magnitude of the response corresponding to the difference between peak and basal concentrations. Each column represents the mean data±s.e.m. calculated from five determinations. **P<0.01.
Kidney International  , DOI: ( /ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions