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P-Selectin Glycoprotein Ligand-1 Contributes to Wound Healing Predominantly as a P- Selectin Ligand and Partly as an E-Selectin Ligand  Hajime Tomita,

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Presentation on theme: "P-Selectin Glycoprotein Ligand-1 Contributes to Wound Healing Predominantly as a P- Selectin Ligand and Partly as an E-Selectin Ligand  Hajime Tomita,"— Presentation transcript:

1 P-Selectin Glycoprotein Ligand-1 Contributes to Wound Healing Predominantly as a P- Selectin Ligand and Partly as an E-Selectin Ligand  Hajime Tomita, Yohei Iwata, Fumihide Ogawa, Kazuhiro Komura, Kazuhiro Shimizu, Ayumi Yoshizaki, Toshihide Hara, Eiji Muroi, Koichi Yanaba, Sangjae Bae, Motoi Takenaka, Minoru Hasegawa, Manabu Fujimoto, Shinichi Sato  Journal of Investigative Dermatology  Volume 129, Issue 8, Pages (August 2009) DOI: /jid Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Macroscopic wound closure. Macroscopic wound closure in wild-type, E-selectin (E-sel)−/−, P-selectin (P-sel)−/−, PSGL-1−/−, and P-sel−/− mice treated with an anti-E-selectin mAb (P-sel−/−+anti-E) at 3 and 7 days after wounding.Representative photographs of open wounds in wild-type and mutant mice at 3 and 7 days after wounding are shown. Full-thickness cutaneous wounds were made using a 6-mm biopsy punch. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Macroscopic and microscopic wound healing parameters in wild-type, E-selectin (E-sel)−/−, P-selectin (P-sel)−/−, PSGL-1−/−, and P-sel−/− mice treated with an anti-E-selectin mAb (P-sel−/−+anti-E) at 3 and 7 days after wounding. (a) The microscopic area of open wound was measured at 3 and 7 days after wounding by tracing of the wound openings onto a transparency. The distance between the migrating edges of keratinocytes under the eschar (the epithelial gap; b) and (c) the area of granulation tissue were both microscopically measured in the tissue sections. (d) Vessel density was microscopically measured in the whole wound bed areas and was expressed as a percentage of the whole wound bed areas. All values represent the mean±SEM. These results were obtained from 14 mice in each group. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Representative inflammatory cell recruitment in the wounded skin from wild-type, E-selectin (E-sel)−/−, P-selectin (P-sel)−/−, PSGL-1−/−, and P-sel−/− mice treated with anti-E-selectin mAb (P-sel−/−+anti-E). (a) Neutrophils were detected in H&E-stained sections at 1 and 4hours after wounding. Arrows show representative neutrophils. Bars: 100μm. (b) Macrophages were detected in anti-F4/80 antibody-stained sections at 3 and 7 days after wounding. Bars: 50μm. (c) Mast cells were detected in toluidine blue-stained sections at 3 and 7 days after wounding. Arrows show mast cells. Bars: 50μm. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Inflammatory cell recruitment in the wounded skin from wild-type, E-selectin (E-sel)−/−, P-selectin (P-sel)−/−, PSGL-1−/−, and P-sel−/− mice treated with an anti-E-selectin mAb (P-sel−/−+anti-E). (a) Numbers of neutrophils per section were counted in entire H&E-stained sections outside the blood vessels under the microscope. (b) Numbers of anti-F4/80 Ab-positive macrophages per field (0.07mm2) were also counted. (c) Numbers of mast cells per section were counted in entire toluidine blue-stained sections. All values represent the mean±SEM. These results were obtained from 10 mice in each group. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Growth factor mRNA expression in the wounded skin tissue from wild type mice, E-selectin (E-sel)−/− mice, P-selectin (P-sel)−/− mice, PSGL-1−/− mice, and P-sel−/− mice treated with anti-E-selectin mAb (P-sel−/−+anti-E) at 3 and 7 days after wounding. Relative mRNA expression of IL-6, IL-10, TNF-α, TGF-β, PDGF, and bFGF was quantified by real-time RT-PCR and normalized relative to endogenous GAPDH levels. All values represent the mean±SEM. These results were obtained from 10 mice in each group. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2009 The Society for Investigative Dermatology, Inc Terms and Conditions

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