Presentation is loading. Please wait.

Presentation is loading. Please wait.

Umbilical Cord Blood Derived Endothelial Progenitor Cells for Tissue Engineering of Vascular Grafts  Dörthe Schmidt, MD, Christian Breymann, MD, Alberto.

Published byEsther Hodges Modified over 5 years ago

Similar presentations

Presentation on theme: "Umbilical Cord Blood Derived Endothelial Progenitor Cells for Tissue Engineering of Vascular Grafts  Dörthe Schmidt, MD, Christian Breymann, MD, Alberto."— Presentation transcript:

1 Umbilical Cord Blood Derived Endothelial Progenitor Cells for Tissue Engineering of Vascular Grafts 
Dörthe Schmidt, MD, Christian Breymann, MD, Alberto Weber, MD, Christina I. Guenter, MD, Stefan Neuenschwander, PhD, Gregor Zund, MD, Marko Turina, MD, Simon P. Hoerstrup, MD  The Annals of Thoracic Surgery  Volume 78, Issue 6, Pages (December 2004) DOI: /j.athoracsur Copyright © 2004 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Example of biodegradable vascular scaffolds (biodegradable polyurethane foam, porosity > 95%, 0.5 cm diameter, 2.5 cm length). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2004 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 (a) Differentiated native endothelial progenitor cells after 3 weeks. (b) Immunofluorescence staining demonstrated the uptake of acetylated low-density lipoprotein (Ac-LDL) (magnification = ×50). (c) von Willebrand factor (vWF) confirms the endothelial phenotype (magnification = ×100). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2004 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Endothelial progenitor cell (EPC) formation on the scaffolds: histology and immunohistochemistry illustrate cell-to-polyurethane foam attachment under static conditions (24 hours after seeding: (a) unseeded scaffold, (b) seeded scaffold, (c) hematoxylin & eosin [HE] staining) and with stable endothelial phenotype of attached EPCs under dynamic conditions on polyurethane foam (48 hours after seeding: (d) cluster of differentiation 31 [CD31], (e) von Willebrand factor [vWF], (f) endothelial nitric oxide synthase [eNOS]) and polyglycolic-acid (PGA) meshes (12 days after seeding: (g) CD31, (h) vWF, (i) HE, magnification = ×20). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2004 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Cell-to-polymer attachment (polyglycolic-acid [PGA] meshes) in the scanning electron microscope (measurement = 70 μm): (a) differentiated endothelial progenitor cell, (b) fiber of polyglycolic-acid/poly(4-hydroxybutric acid) (PGA/P4HB) scaffold. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2004 The Society of Thoracic Surgeons Terms and Conditions

Download ppt "Umbilical Cord Blood Derived Endothelial Progenitor Cells for Tissue Engineering of Vascular Grafts  Dörthe Schmidt, MD, Christian Breymann, MD, Alberto."

Similar presentations

Perioperative Statin Therapy for Patients Undergoing Coronary Artery Bypass Grafting  Amr F. Barakat, MD, Marwan Saad, MD, PhD, Ahmed Abuzaid, MD, Amgad.

Perioperative Statin Therapy for Patients Undergoing Coronary Artery Bypass Grafting  Amr F. Barakat, MD, Marwan Saad, MD, PhD, Ahmed Abuzaid, MD, Amgad.
Resveratrol, a Natural Red Wine Polyphenol, Reduces Ischemia-Reperfusion–Induced Spinal Cord Injury  Sadi Kaplan, MD, Gianluigi Bisleri, MD, Jeffrey A.

Resveratrol, a Natural Red Wine Polyphenol, Reduces Ischemia-Reperfusion–Induced Spinal Cord Injury  Sadi Kaplan, MD, Gianluigi Bisleri, MD, Jeffrey A.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Squamous Cell Carcinoma of the Pericardium Found During Coronary Bypass Surgery of Possible Cutaneous Origin  Alan P. Kypson, MD, Curtis A. Anderson,

Squamous Cell Carcinoma of the Pericardium Found During Coronary Bypass Surgery of Possible Cutaneous Origin  Alan P. Kypson, MD, Curtis A. Anderson,
Use of Human Umbilical Cord Blood-Derived Progenitor Cells for Tissue-Engineered Heart Valves  Ralf Sodian, MD, Philipp Schaefermeier, PhD, Sybille Abegg-Zips,

Use of Human Umbilical Cord Blood-Derived Progenitor Cells for Tissue-Engineered Heart Valves  Ralf Sodian, MD, Philipp Schaefermeier, PhD, Sybille Abegg-Zips,
Bing-Yen Wang, MD, Hsiao-Huang Chang, MD, PhD, I

Bing-Yen Wang, MD, Hsiao-Huang Chang, MD, PhD, I
In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery  Chao Yang, MD, Ralf Sodian, MD, Ping.

In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery  Chao Yang, MD, Ralf Sodian, MD, Ping.
Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,

Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,
Tissue Engineering of Autologous Human Heart Valves Using Cryopreserved Vascular Umbilical Cord Cells  Ralf Sodian, MD, Cora Lueders, PhD, Liv Kraemer,

Tissue Engineering of Autologous Human Heart Valves Using Cryopreserved Vascular Umbilical Cord Cells  Ralf Sodian, MD, Cora Lueders, PhD, Liv Kraemer,
Perioperative Statin Therapy for Patients Undergoing Coronary Artery Bypass Grafting  Amr F. Barakat, MD, Marwan Saad, MD, PhD, Ahmed Abuzaid, MD, Amgad.

Perioperative Statin Therapy for Patients Undergoing Coronary Artery Bypass Grafting  Amr F. Barakat, MD, Marwan Saad, MD, PhD, Ahmed Abuzaid, MD, Amgad.
Tracheal Replacement With a Silicone-Stented, Fresh Aortic Allograft in Sheep  Hisashi Tsukada, MD, PhD, Armin Ernst, MD, Sidhu Gangadharan, MD, Simon.

Tracheal Replacement With a Silicone-Stented, Fresh Aortic Allograft in Sheep  Hisashi Tsukada, MD, PhD, Armin Ernst, MD, Sidhu Gangadharan, MD, Simon.
Fariba Chalajour, MD, Laura A

Fariba Chalajour, MD, Laura A
Chest Wall Angiolipoma Complicating Von Recklinghausen Disease

Chest Wall Angiolipoma Complicating Von Recklinghausen Disease
Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells  Paul DiMuzio, MD, Thomas Tulenko, PhD  Journal.

Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells  Paul DiMuzio, MD, Thomas Tulenko, PhD  Journal.
Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve  Simon P Hoerstrup, MD, Gregor Zünd, MD, Andreina.

Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve  Simon P Hoerstrup, MD, Gregor Zünd, MD, Andreina.
Engineering Patient-Specific Valves Using Stem Cells Generated From Skin Biopsy Specimens  David L. Simpson, PhD, Brody Wehman, MD, Yekaterina Galat,

Engineering Patient-Specific Valves Using Stem Cells Generated From Skin Biopsy Specimens  David L. Simpson, PhD, Brody Wehman, MD, Yekaterina Galat,
Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,

Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Dhruva K. Mishra, PhD, Michael J. Thrall, MD, Brandi N

Dhruva K. Mishra, PhD, Michael J. Thrall, MD, Brandi N
Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD,

Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD,

Similar presentations

Ads by Google