1. Volume 144, Issue 2, Pages 294-297 (February 2013)
Optical Imaging of Periostin Enables Early Endoscopic Detection and Characterization of Esophageal Cancer in Mice 
Gabrielle S. Wong, Peiman Habibollahi, Pedram Heidari, Ju–Seog Lee, Andres J. Klein–Szanto, Todd J. Waldron, Phyllis Gimotty, Hiroshi Nakagawa, Philip R. Taylor, Timothy C. Wang, Umar Mahmood, Anil K. Rustgi 
Gastroenterology 
Volume 144, Issue 2, Pages (February 2013)
DOI: /j.gastro
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2. Figure 1
Periostin is up-regulated in invasive human ESCC. (A) Schematic of prediction models constructed for evaluation of predicted outcomes based on gene expression signatures. (B) Kaplan–Meier plots of the overall survival (OS) of the 2 predicted groups of patients. The differences between groups were significant, as indicated by the log-rank test. (C) Comparison of relative periostin (POSTN) expression in gene expression dataset comparing invading vs noninvading EPC2-hTERT-EGFR-p53R175H cells grown in organotypic culture (OTC) to periostin expression in the gene expression dataset of matched ESCC tumors in the Shanxi cohort. Error bars represent ± standard error of the mean. *P < .05. (D) Immunoblot of POSTN (95 kilodaltons) expression in the panel of ESCC cell lines. β-actin was used as a loading control. (E) Fluorescent images of the periostin optical probe injected into nude mice bearing tumor xenografts of TE-11 and TT (white arrows). TT tumor xenografts were used as a negative control. The sternum is indicated by black arrowheads and the bladder is indicated by black arrows. (F) Quantification of fluorescent signal from the periostin optical probe detected from TE-11 and TT tumor xenografts (n = 5 from each group). Bar graphs represent target-to-background ratios ± standard error of the mean. *P < .05. Note that a background target-to-background ratio of 1 implies no increased probe uptake in tumor relative to background tissue.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2013 AGA Institute Terms and Conditions

3. Figure 2
Periostin expression is detected with increasing ESCC progression in the genetic mouse model of ESCC. (A) Immunoblot of periostin expression in mouse sera from 3 cohorts of mice at different stages of disease: control L2-cre; p120ctnLoxP/+, L2-cre; p120ctnLoxP/LoxP with mild dysplasia and L2-cre; p120ctnLoxP/LoxP with severe dysplasia (n = 6 mice from each group). Ponceau S staining of immunoblot was performed as loading control. (B) Enzyme-linked immunosorbent assay of periostin levels present in mouse sera from the earlier-described 3 cohorts of mice (n = 6 mice from each cohort). Error bars represent ± standard error of the mean. *P < .05. (C) Representative WL, NIR, and overlay images from upper-gastrointestinal endoscopies in mice from the earlier-described 3 cohorts. Color map represents the range of fluorescent signal intensities (200–1100) in images. (D) Quantification of the fluorescent signal from the periostin optical probe detected from upper-gastrointestinal endoscopies in mice from the earlier-described 3 cohorts (n = 3 from each cohort). Bar graphs represent target-to-background ratio ± standard error of the mean. *P < .05. (E) Histopathologic analysis of representative esophagi isolated from mice from the earlier-described 3 cohorts in top panels to bottom panels. Scale bars: 100 μmol/L.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2013 AGA Institute Terms and Conditions