1. Chromatin binding sites shared by pCREB1 and ERα are predominantly cAMP induced.
Chromatin binding sites shared by pCREB1 and ERα are predominantly cAMP induced. (A) Heat maps showing the ChIP-seq signals of pCREB1 over the ERα binding sites in response to the indicated stimuli. Regions are sorted according to decreasing signals in pCREB1 binding. Data are centered at the ERα peaks with a 500-bp window on either side. All data are from MDA-MB-134 cells. (B, C) Venn diagrams of the cAMP-induced sites (B) and the E2-induced sites (C) indicating those shared between ERα (red) and pCREB1 (blue). (D) Genomic distribution of the peaks shared between ERα and pCREB1 in response to the indicated treatments. (E) Snapshots from the ChIP-seq data for both ERα and pCREB1 peaks are shown across indicated genes for DMSO (blue), E2 (red), and FI (green). Genomic coordinates are indicated. The numbers in square brackets in each panel indicate the scale based on the numbers of reads.
Melissa Berto et al. LSA 2018;1:e
© 2018 Berto et al.