1. Volume 39, Issue 1, Pages 184-195 (July 2013)
γδ T Cells Exhibit Multifunctional and Protective Memory in Intestinal Tissues 
Brian S. Sheridan, Pablo A. Romagnoli, Quynh-Mai Pham, Han-Hsuan Fu, Francis Alonzo, Wolf-Dieter Schubert, Nancy E. Freitag, Leo Lefrançois 
Immunity 
Volume 39, Issue 1, Pages (July 2013)
DOI: /j.immuni
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2. Immunity 2013 39, 184-195DOI: (10.1016/j.immuni.2013.06.015)
Copyright © 2013 Elsevier Inc. Terms and Conditions

3. Figure 1
A Distinct Subset of Lm-Elicited γδ T Cells Transitions into the Memory Phase and Recalls to a Secondary Challenge
(A and B) Representative contour plots are gated on γδ T cells (identified by staining with GL3, a Cδ TCR-specific mAb) from MLNs (A) or pLNs (B) on the indicated day postinfection (dpi). Values represent the percentage of cells within the indicated gates. See also Figure S1.
(C) Representative histograms gated on total naive γδ T cells or indicated subsets from the MLNs of 10 dpi mice were analyzed for expression of the indicated Vγ or Vδ regions. Values corresponding to the percentage of cells within the gated population are shown.
(D) Two distinct gating strategies identify Lm-elicited γδ T cells. Representative contour plots of γδ T cells from 9 dpi MLNs were analyzed for CD27, CD44, Vγ1.1, Vγ2, Vγ3, Vγ5, and CD8α. Values corresponding to the percentage of cells within the gated population are shown.
(E) 17D1 identifies Vγ4Vδ1-expressing T cells after preincubation with GL3. γδ T cells from the MLNs of memory mice and memory mice given a secondary oral infection were characterized for CD27 and CD44 expression and 17D1 reactivity. Red contour represents CD27− CD44hi γδ T cells, and blue contour represents CD27+ γδ T cells.
Immunity  , DOI: ( /j.immuni )
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4. Figure 2
Mobilization of Lm-Elicited γδ T Cells Results in Accumulation within Intestinal Lamina Propia, but Not within the Epithelium
(A and B) Blood was collected from Lm-infected mice and analyzed for GL3, Vγ chain (A), and α4β7 (B) expression. Mice (three to five per group) were given a secondary Lm infection at 30 dpi and were monitored for an additional 11 days. Subsets labeled Vγ4 specifically refer to γδ T cells, which do not express Vγ1.1, Vγ2, Vγ3, or Vγ5. Data represent the mean ± SEM. See also Figure S2A.
(C) Representative contour plots are gated on γδ T cells from pLNs, MLNs, IELs, and the lamina propia (LP) at the indicated times after infection. Values represent the percentage of cells within the indicated gates. Representative contour plots are shown from multiple experiments with three to four mice per group.
(D) Representative contour plots gated on Vγ4+ CD8α− γδ T cells (as indicated in C) from the MLNs and LP were characterized for expression of CD44 and CD27 or CD127 and CD103. See also Figure S2B.
Immunity  , DOI: ( /j.immuni )
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5. Figure 3
Lm-Elicited γδ T Cells Display Contextual Specificity to the Priming Pathogen
(A) Mice infected 170 days previously were challenged either by oral (po) infection with 2 × 1010 CFU of Lm or by i.v. infection with 2 × 105 CFU of Lm. MLNs were harvested at the indicated times after secondary infection for the enumeration of γδ T cells on the basis of CD27 and CD44 expression. Data are displayed as the mean ± SEM and are representative of at least two independent experiments with three to four mice per group. See also Figures S3A–S3D.
(B) Mice that had received a secondary infection were rested an additional 124 days prior to a tertiary oral Lm infection with 2 × 1010 CFU of Lm, and γδ T cells were analyzed for CD27 and CD44 expression 5 days later (red squares represent individual mice). Data represent the mean ± SEM.
(C and D) γδ T cells from the MLNs of oral Lm-immune mice or oral Lm-immune mice rechallenged by oral infection with Lm, S. typhimurium strain BRD509, or S. typhimurium strain SL1344 were analyzed 5 days later for CD27 and CD44 expression (C) and α4β7 expression on CD27− CD44hi γδ T cells (D). Representative data are displayed as the mean ± SEM from two independent experiments with three to four mice per group. See also Figure S3E.
Immunity  , DOI: ( /j.immuni )
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6. Figure 4
Evolution of the Cytokine Response from Lm-Elicited γδ T Cells
(A and B) MLNs from naive mice or mice infected with Lm were stimulated with αCD3- and αCD28-coated beads for 5 hr in the presence of brefeldin A. γδ T cells were analyzed for CD27, CD44, IL-17A, and IFN-γ after stimulation. Representative dot plots from three to five mice per group and at least two experiments are displayed in (A) and represent the mean ± SEM in (B). See also Figure S4.
(C and D) MLNs from Lm-infected mice were stimulated with beads as described in (A). γδ T cells were analyzed for IL-17A (C) and IFN-γ (D) after stimulation. Representative dot plots show the total CD3+ cells from three to five mice per group and values of the percentage of cells within gated quadrants (upper panels). GL3 expression was determined from histograms gated on IL-17A (C)- or IFN-γ (D)-producing T cells from the MLNs of infected mice. The numbers within the histograms refer to the percentage of cells within the indicated gates.
(E) MLNs were harvested from infected mice, and γδ T cell subsets were analyzed for intracellular expression of T-bet and RORγt. Representative histograms are shown for experiments with four to five mice per group.
Immunity  , DOI: ( /j.immuni )
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7. Figure 5
MHC-II-Expressing Cells Substantially Contribute to Lm-Elicited γδ T Cell Effector Functions
(A–D) A total of 1 × 106 MLNs from Lm-immune Thy1.2 mice (A and B) or Lm-immune Thy1.2 mice 5 days after secondary oral Lm infection (C and D) were mixed with 1 × 106 MLNs from Thy1.1 congenic mice as indicated above the contour plots or were left unstimulated. IL-17A and IFNγ production was determined by intracellular cytokine staining of Thy1.2+ γδ T cells after 5 hr in vitro in the presence of brefeldin A. Red contour represents CD27− CD44hi Thy1.2+ γδ T cells, and blue contour represents CD27+ Thy1.2+ γδ T cells. The values in the contour plots represent the percentage of cytokine-producing cells from CD27− CD44hi γδ T cells. Data are representative of at least two independent experiments.
Immunity  , DOI: ( /j.immuni )
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8. Figure 6
γδ T Cells Collaborate with αβ T Cells to Protect against Secondary Infection
Naive mice or Lm-immune mice treated with the indicated combinations of GK1.5 (αCD4), 2.43 (αCD8), GL4 (αTCRδ), 2.11 (αVγ1.1), and UC3-10A6 (αVγ2) were orally infected with 2 × 1010 CFU of Lm. Bacterial burden was enumerated in the MLNs and small intestine 4 days later. Data are presented as the mean ± SEM from 5–15 mice per group. ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < p values > 0.05 are indicated by “ns” (nonsignificant). See also Figure S5.
Immunity  , DOI: ( /j.immuni )
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