1. Untreated Aphidicolin Wt Ctrl Wt Cre Atrflox Ctrl Atrflox Cre BRDU
S/G2/M: 69% + 2
BrdU+: 3% + 0.2
S/G2/M: 47% + 1
Wt Ctrl
BrdU+: 76% + 5
S/G2/M: 73% + 4
BrdU+: 3% + 2
S/G2/M: 47% + 1
Wt Cre
BrdU+: 72% + 8
S/G2/M: 68% + 1
BrdU+: 3% + 0.4
S/G2/M: 46% + 3
Atrflox Ctrl
BrdU+: 71% + 5
S/G2/M: 66% + 3
BrdU+: 1.2% + 0.1
S/G2/M: 47% + 1
Atrflox Cre
BRDU
7-AAD
Supplementary Figure S3. Cell cycle status of ATR-deleted fibroblasts. Subconfluent fibroblast cultures from Atrflox/- mice or wildtype B6 mice were transduced with MSCV-IRES-GFP or MSCV-Cre-IRES-GFP. Cells were cultured for 4 days, splitting as necessary, and replated for 1 day before treatment with 4 M aphidicolin. Before treatment and 16 hours after addition of aphidicolin cells were labeled with 10 M BrdU for 75 minutes and examined for BrdU incorporation and DNA content as described (Tough, D. F. & Sprent, J J. Exp. Med. 179, 1127–1135 (1994)).
Raulet et al (MS B-Z)