1. Volume 133, Issue 4, Pages 1250-1260 (October 2007)
Clock Gene Expression in the Murine Gastrointestinal Tract: Endogenous Rhythmicity and Effects of a Feeding Regimen 
Willemijntje A. Hoogerwerf, Helen L. Hellmich, Germaine Cornélissen, Franz Halberg, Vahakn B. Shahinian, Jonathon Bostwick, Tor C. Savidge, Vincent M. Cassone 
Gastroenterology 
Volume 133, Issue 4, Pages (October 2007)
DOI: /j.gastro
Copyright © 2007 AGA Institute Terms and Conditions

2. Figure 1
(A) Agarose gel showing RT-PCR products for parts of individual clock gene coding regions. Left lane shows the marker. No-RT controls are flanking clock gene amplimers to the right. Predicted amplimers sizes: clock, 491 bp; per1, 200 bp; per2, 991 bp; per3, 527 bp; cry1, 551 bp; cry2, 600 bp; bmal1, 992 bp. (B) Images of distal colon with hematoxylin counter staining (original magnification, ×40). Thin black arrows point toward areas of positive (brown) staining cells in the myenteric plexus, and thick black arrows point toward areas of positive staining of epithelial cells for PER2 antibody and BMAL antibody. (C) Images of double labeling immunohistochemical staining of PER1 and PER2 (green) with β-tubulin (red) in the murine colon. The overlap image represents superimposition of fluorescent and bright field images. LM, longitudinal muscle; CM, circular muscle, MP, myenteric plexus. Bar, 20 μm. Arrows point toward area of overlapping staining in the myenteric plexus.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

3. Figure 2
Daily profiles of colonic clock gene mRNA levels and corresponding fitted cosinor curves. The mRNA levels are expressed as means ± SE (n = 3 mice per time point). PR, percentage rhythm; P value, probability from zero-amplitude (no rhythm) test.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

4. Figure 3
Acrophase of distal colonic clock genes for mice with ad libitum food access (solid squares) and following 48 hours of timed feeding (open squares). Bars represent 95% confidence intervals. Grey shade indicates dark cycle.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

5. Figure 4
(A) Western blotting for clock proteins (BMAL and PER2) in mice (n = 3 mice per time point) with ad libitum access to food and following 48 hours of timed feeding. (B) Quantitative analysis of clock gene protein rhythms over a 24-hour time span and corresponding fitted cosinor curves.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions

6. Figure 5
(A–C) Cresyl violet stained coronal section of the mouse brain (original magnification, ×20). Arrows delineate the SCN, flanking the third ventricle, before LCM (A) and after LCM (B). Panel C shows SCN on cap. (D) Acrophase for bmal and per2 in the SCN for mice with ad libitum access to food and following 48 hours or 1 week of timed feeding. Solid box represents bmal; diamond-shaped box represents per2. Bars represent SE. Grey shade indicates dark cycle.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2007 AGA Institute Terms and Conditions