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Reassembling Biological Machinery In Vitro

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1 Reassembling Biological Machinery In Vitro
Henry Hess  Chemistry & Biology  Volume 16, Issue 9, Pages (September 2009) DOI: /j.chembiol Copyright © 2009 Elsevier Ltd Terms and Conditions

2 Figure 1 Controlled Immobilization of Two Enzymes of the Glycolytic Cycle Two genetically engineered enzymes, hexokinase (HK) and glucose-6-phosphate isomerase (GPI), linked to a functionalized surface via His6-tag/Ni-NTA interactions, perform the first two steps of the glycolytic pathway conversion of glucose to glucose-6-phosphate catalyzed by HK, followed by subsequent transformation of glucose-6-phosphate to fructose-6-phosphate, catalyzed by GPI. Chemistry & Biology  , DOI: ( /j.chembiol ) Copyright © 2009 Elsevier Ltd Terms and Conditions


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