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Volume 1, Issue 2, Pages (March 2008)

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1 Volume 1, Issue 2, Pages 285-294 (March 2008)
Characterization of Gibberellin Receptor Mutants of Barley (Hordeum vulgare L.)  Chandler Peter M. , Harding Carol A. , Ashton Anthony R. , Mulcair Mark D. , Dixon Nicholas E. , Mander Lewis N.   Molecular Plant  Volume 1, Issue 2, Pages (March 2008) DOI: /mp/ssn002 Copyright © 2008 The Authors. All rights reserved. Terms and Conditions

2 Figure 1 BestFit (GCG) Analysis of Deduced Amino Acid Sequences of Barley GSE1 (upper line) and Rice GID1 (lower line). The bold characters in grey highlight represent sites of missense substitutions (see Table 1 for details of the barley mutants, and Ueguchi-Tanaka et al. (2007) for the rice mutants). An intron occurs in both species between the codons for K13 and T14. Molecular Plant 2008 1, DOI: ( /mp/ssn002) Copyright © 2008 The Authors. All rights reserved. Terms and Conditions

3 Figure 2 Phylogenetic Tree of the Relationship of GA Receptors and Putative Orthologues to Closely Related Esterases. The tree includes the five closest esterase relatives to the GA receptors in the Arabidopsis and rice genomes shown by their respective genome initiative numbers. The sequences from other species are shown by a species abbreviation. The sequence accession numbers with abbreviations are: Actde_GID1, ABB89021, Actinidia deliciosa; Vitvi_GID1, CAN65915, Vitis vinifera; Goshi_GID1a, ABG89394, Goshi_GID1b, ABQ96123, Gossypium hirsutum; Medtr_GID1a, BN001191, Medtr_GID1b, BN001192, Medicago truncatula; Gosra_GID1a, CO , Gosra_GID1b, CO , Gossypium raimondi; Aqux_GID1, BN001198, Aquilegia formosa × Aquilegia pubescens; Lyces_GID1, BN001197, Lycopersicon esculentum; Allce_GID1a, BN001199, Allce_GID1b BN001200, Allium cepa; Triae_GID1, BN001201, Triticum aestivum; Zeama_GIDa, BN001194, Zeama_GID1b, BN001195, Zea mays; Sorbi_GID1, BN001196, Sorghum bicolor; Sacof_GID1, BN001193, Saccharum officinarum; Pinta_GID1, BN001190, Pinus taeda; Picgl_GID1, BN001188, Picea glauca. The tree was generated by the Neighbour-Joining method using the MEGA 4 program (Tamura et al., 2007). Molecular Plant 2008 1, DOI: ( /mp/ssn002) Copyright © 2008 The Authors. All rights reserved. Terms and Conditions

4 Figure 3 In Vitro Binding of Radiolabelled GAs to Expressed GSE1 Protein. [14C] radio-labelled GAs with or without protein (GSE1 or ovalbumin) or a 100-fold molar excess of [12C] GA were subject to gel filtration to separate protein-bound GA from free GA. Molecular Plant 2008 1, DOI: ( /mp/ssn002) Copyright © 2008 The Authors. All rights reserved. Terms and Conditions

5 Figure 4 Growth Responses of gse1 Mutants to Exogenous GA3.
(A) LERmax of control (no GA3) seedlings, and the increment in LERmax in response to 1 mM GA3. (B) Partitioning of the growth increment into the percentages that occur in the range 0–0.01 mM, and 0.01–1 mM. Molecular Plant 2008 1, DOI: ( /mp/ssn002) Copyright © 2008 The Authors. All rights reserved. Terms and Conditions

6 Figure 5 Production of α-amylase by Endosperm Half-Grains of Himalaya and Four gse1 Mutants after 3 d in Different Concentrations of Exogenous GA3. Molecular Plant 2008 1, DOI: ( /mp/ssn002) Copyright © 2008 The Authors. All rights reserved. Terms and Conditions


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