1. A B C
AR-V7/AR-V7
ARv567es/ARv567es * *
VC-
AR-V7
ARv567es- VC
BiFC
Relative intensity
BiFC
Relative intensity
AR-V7-VN
ARv567es-VN
Mock Ctrl
D-mut
D-mut
wt AR-V7-VN
Hoechst
wt ARv567es-VN
Hoechst wt wt
AR-FL
ARv567es
AR-V7
merged
merged
GAPDH wt
D-mut wt
D-mut
VC-AR-V7
ARv567es-VC
Figure S5. BiFC detection of AR-V/AR-V dimerization in C4-2 cells. (A & B) Dimerization was detected by the BiFC assay in C4-2 cells under androgen-deprived condition. Right panels, quantitation of BiFC signals by flow cytometry. wt, wild-type; D-mut, D-box mutant. Hoechst, nuclear stain. Scale bars, 10 µm. *, P < (C) Western blotting with a pan-AR antibody showing BiFC-AR-V fusion proteins are expressed at a much lower level than the endogenous AR-FL.