1. Volume 4, Issue 6, Pages 1052-1061 (November 2011)
The Psb32 Protein Aids in Repairing Photodamaged Photosystem II in the Cyanobacterium Synechocystis 6803 
Wegener Kimberly M. , Bennewitz Stefan , Oelmüller Ralf , Pakrasi Himadri B.  
Molecular Plant 
Volume 4, Issue 6, Pages (November 2011)
DOI: /mp/ssr044
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

2. Figure 1 Conservation of the Psb32 Protein in Photosynthetic Organism.
Star indicates Psb32 in Synechocystis Colors indicate taxonomy: blue, cyanobacteria; green, eukaryotic plants; brown, green algae; red, red algae. The tree was rooted using the eukaryotes as the outgroup. The numbers at the branch points represent bootstrap values as a percentage from 100 replications. The protein identification numbers used to generate the tree are listed in Supplemental Figure 1.
Molecular Plant 2011 4, DOI: ( /mp/ssr044)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

3. Figure 2 Western Blots Showing the Subcellular Localization of Psb32
(A) Total cellular membranes were isolated and then separated by polyethylene glycol dextran two-phase partitioning to isolate thylakoid membrane and plasma membrane fractions.
(B) Total cellular membranes were treated with 1% Triton X-114 to release loosely associated proteins from the membrane.
Molecular Plant 2011 4, DOI: ( /mp/ssr044)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

4. Figure 3 Genetic Deletion of psb32
(A) Scheme of psb32 deletion by a chloramphenicol resistance cassette (CmR).
(B) PCR confirmation of the segregation of Δpsb32 mutation.
(C) RT–PCR to evaluate expression of psb32, the downstream gene sll1866, and the control RNaseP gene in Δpsb32 mutant in comparison to WT.
Molecular Plant 2011 4, DOI: ( /mp/ssr044)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

5. Figure 4 Growth under Various Oxidative Stresses.
Open shapes indicate WT; closed shapes indicate Δpsb32
(A) Growth of WT and Δpsb32 in the presence of H2O2.
(B) Growth of WT and Δpsb32 in the presence of 7.5 μM Rose Bengal and 200 μmol photons m−2 s−1 white light. Error bars indicate standard deviation of biological replicates, n = 3.
Molecular Plant 2011 4, DOI: ( /mp/ssr044)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Competition between WT and Δpsb32 under Low Light, Growth Light, and High Light
(A) At each time point, genomic DNA was isolated and PCR conducted to determine the relative abundance of each strain. A representative gel is shown.
(B) Quantification of the PCR band intensity of the high light grown samples. White circles indicate WT; black circles indicate Δpsb32. Error bars indicate standard deviation of biological replicates, n = 3.
Molecular Plant 2011 4, DOI: ( /mp/ssr044)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
psb32 Mutant Displays Increased Photoinhibition and Decreased Recovery.
White circles indicate WT; black circles indicate Δpsb32
(A) Cells were incubated in the presence of lincomycin and high light for 60 min. Fv/Fm was measured every 30 min.
(B) Cells were washed and allowed to recover in BG11 under low light. Fv/Fm was measured every 30 min. Error bars indicate standard deviation of biological replicates, n = 3.
Molecular Plant 2011 4, DOI: ( /mp/ssr044)
Copyright © 2011 The Authors. All rights reserved. Terms and Conditions