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Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture.

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Presentation on theme: "Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture."— Presentation transcript:

1 Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized guinea pigs  M.-W. Dai, J.-G. Chu, F.-M. Tian, H.-P. Song, Y. Wang, Y.-Z. Zhang, L. Zhang  Osteoarthritis and Cartilage  Volume 24, Issue 6, Pages (June 2016) DOI: /j.joca Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 Macroscopic view of right tibial plateau of samples and OARSI macroscopic scoring between groups. (A) Sham group, (B) MNX group, (C) CLX group, (D) PTH group, and the macroscopic scores were illustrated in (E). Medial tibial plateau cartilage surface of animals undergoing MNX operation became rougher and larger than in the Sham group, with cleft and chondral hyperplasia forming on the medial edge of the cartilage. Black arrows indicate lesions of tibial plateau, white arrows indicate osteophytes on the edge of the tibial plateau, and the black arrow head indicates clefts on the tibial plateau. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 OARSI Microscopic scoring between groups (toluidine blue staining, 40×) toluidine blue stain of medial tibial plateau of samples. (A) Sham group, (B) MNX group, (C) CLX group, and (D) PTH group. The results of scoring subsets were illustrated in (E–I), and the total scores were illustrated in (J), and the results of cartilage thickness were illustrated in (K). Bars represent length of 1 mm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

4 Fig. 3 Immunohistochemical staining of groups. (100×) This figure demonstrated the expression of (A) AGG, (B) Col-2, (C) caspase-3, (D) ADAMTS4, (E) MMP-13, and (F) periostin in load-bearing areas of cartilage among groups. Bars represent a length of 400 μm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

5 Fig. 4 Analysis of immunohistochemical staining. A–F show the quantified protein levels of (A) AGG, (B) Col-2, (C) caspase-3, (D) ADAMTS4, (E) MMP-13, and (F) periostin in load-bearing areas of cartilage among groups, and G–I show the ratio of (G) AGG/ADAMTS4, (H) Col-II/MMP-13, (I) and Col-II/AGG between groups. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

6 Fig. 5 2D reconstruction of coronal section of right tibial plateau from each group. (A) Sham group, (B) MNX group, (C) CLX group, and (D) PTH group. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions


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