1. 2014 GEM Midwest Trial Results & New Releases
Germplasm Enhancement of Maize
C. Gardner, F. Engstrom, M. Peters, M. Shen
USDA-ARS NCRPIS GEM
- December 10, 2014

2. Commercial Checks 2014 Germplasm Enhancement of Maize Midwest RM
Hybrid
Midwest RM
HC33xLH287
109
Pioneer 34R65
109
MBS3520
hybrids
MBS8814
hybrids
MBS3520/MBS8814
112
Pioneer 33F85
114
LH200xLH262
118
Pioneer 31D58
119
Germplasm Enhancement of Maize

4. 2015 GEM Releases from Ames GEM Code Pedigree Race Type Het Grp
GEMN-0260
(GEMN-0140/GEMN-0097)-B-B-026-B-B
Dente Amarillo/FS8BT
12.5% Tropical NS
GEMN-0261
(GEMN-0140/GEMN-0097)-B-B-027-B-B
GEMS-0262
(GEMS-0147/GEMS-0180)-B B-B
Suwan/Tusón SS
GEMS-0263
BR105:S1626(GEMS-0175)-B-049-B-B
Composite Suwan/Tusón
GEMS-0264
BR105:S B-B
Composite Suwan
25% Tropical
GEMS-0265
DKXL212:S0950-B-083-B-B
Hybrid Tropical
GEMN-0266
NEI9008:N0826(GEMN-0155)-B-074-B-B
Suwan/Cristalino Colorado
GEMN-0267
NEI9008:N0826(GEMN-0155)-B-095-B-B
GEMS-0268
NS1:S0852-B-021-B-B
Suwan
GEMS-0269
NS1:S0852-B-054-B-B

5. GEMN-0260 (GEMN-0140/GEMN-0097)-B-B-026
Tester: HC33
Tester: MBS3520
GEM Code
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
% Yd /CHK
GEMN-0260
(GEMN-0140/GEMN-0097)-B-B-026 17
183
20.0
9.1
55.0
1.2
7.8 3
161
89.2
18.5
8.8
53.5
2.4
3.0
Test Entry Means
157
19.2
8.2
55.9
4.4
20.4
7.9
54.2
3.1
9.7
Check Means
191
21.1
9.0
7.0
181
21.8
8.3
55.2
1.9
4.9
Overall Means
19.5
4.7
164
20.6
8.0
54.4 CV 14 13
6.6
F-value
5.0
7.2
6.0
LSD (p=0.05)
26.4
1.6 23
1.5
Germplasm Enhancement of Maize
Better roots than GEMN-0261;Dente Amarelo (Brazil) x Mixed;

6. GEMN-0261 (GEMN-0140/GEMN-0097)-B-B-027
Tester: HC33
Tester: MBS3520
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
(GEMN-0140/GEMN-0097)-B-B-027 16
175
19.4
9.0
55.0
0.4
2.2 4
183
20.0
9.2
53.9
1.9
21.2
Test Entry Means
157
19.2
8.2
55.9
2.4
4.4
161
20.4
7.9
54.2
3.1
9.7
Check Means
191
21.1
7.0
181
21.8
8.3
55.2
4.9
Overall Means
19.5
4.7
164
20.6
8.0
54.4
3.0 CV
14.2
13.1
6.6
F-value
5.0
7.2
6.0
LSD (p=0.05)
26.4
1.6
23.6
1.5
Germplasm Enhancement of Maize
Hybrid Tropical – Brazil; more consistent yield than GEMN-0260

7. GEMS-0262 (GEMS-0147/GEMS-0180)-B-104-001
Tester: LH287
Tester MBS8814
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
(GEMS-0147/GEMS-0180)-B 29
202
24.0
8.5
52.6
0.8
8.1 12
198
25.1
7.9
51.6
0.6
3.9
Test Entry Means
179
23.2
7.7
53.3
3.5
10.8
183
23.3
53.7
1.4
4.9
Check Means
194
22.2
8.8
55.9
5.1
196
22.6
8.7
55.3
1.8
10.1
Overall Means
180
23.1
7.8
53.6
3.3
10.2
184
8.0
53.9
5.4 CV
9.3
4.3
6.3
F-value
3.2
10.4
2.2
LSD (p=0.05)
20.4
1.2
21.5
1.6
Germplasm Enhancement of Maize
Thai tropical inbred x Tuson (Guatemala)

8. GEMS-0263 BR105:S1626(GEMS-0175)-B-049 Germplasm Enhancement of Maize
Tester: MBS9508
Tester: LH287
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
BR105:S1626(GEMS-0175)-B-049 43
158
21.4
7.4
58.8
0.4
8.1 14
202
24.6
8.2
53.4
2.1
4.3
Test Entry Means
135
22.0
6.2
58.2
4.6
10.3
186
23.3
8.0
1.9
7.8
Check Means
134
21.5
6.3
56.9
1.0
8.5
198
22.1
9.0
56.3
1.6
2.9
Overall Means
187
23.2
53.7 CV
15.7
9.2
F-value
1.5
8.3
2.8
15.3
LSD (p=0.05)
30.4
1.3
17.2
Germplasm Enhancement of Maize
Composite Suwan (Brazil) x Tuson (Br. Virgin Islands)

9. GEMS-0264 BR105:S1643-078-001 Germplasm Enhancement of Maize
Tester: AGR-nSS
Tester: LH287
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
BR105:S 43
169.2
20.5
8.3
56.3
0.0
9.4 36
204.3
24.7
52.4
1.4
7.9
Test Entry Means
151.5
21.1
7.2
55.6
1.0
186.2
23.3
8.0
53.4
1.9
7.8
Check Means
162.1
19.6
56.2
0.2
3.3
198.9
22.1
9.0
1.6
2.9
Overall Means
187.3
23.2
8.1
53.7
7.4 CV
15.1
6.9
9.2
4.3
F-value
2.4
4.1
2.8
15.3
LSD (p=0.05)
29.7
1.8
17.2
Germplasm Enhancement of Maize
Suwan (Thailand); top of test with LH287 tester; equal maturity to LH200/LH262 check; much shorter ear ht; better roots than others in this family

10. GEMS-0265 DKXL212:S0950-B-083 Germplasm Enhancement of Maize
Tester: LH287
Tester: MBS8814
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
DKXL212:S0950-B-083 32
196
22.3
8.8
54.7
1.2
2.6
189
22.8
8.3
52.4
1.4
0.5
Test Entry Means
186
23.3
8.0
53.4
1.9
7.8
180
22.7
51.9
1.3
4.0
Check Means
198
22.1
9.0
56.3
1.6
2.9
21.4
8.7
54.1
1.7
2.7
Overall Means
187
23.2
8.1
53.7
7.4
181
22.6
52.1
3.9 CV
9.2
4.3
8.2
5.7
F-value
2.8
15.3
3.2
10.5
LSD (p=0.05)
17.2
1.0
14.9
Germplasm Enhancement of Maize
MBS8815 tester in previous year also had an excellent record. Hybrid tropical from Brazil. Equal in maturity to 33F85

11. GEMN-0266 NEI9008:N0826(GEMN-0155)-B-074
Tester: HC33
Tester: MBS3520
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
NEI9008:N0826(GEMN-0155)-B-074 13
179
20.3
8.9
54.3
3.3
13.3 11
201
24.2
8.3
53.4
1.8
9.8
Test Entry Means
156
19.8
7.9
54.9
3.7
6.0
172
22.3
7.7
53.9
2.0
9.0
Check Means
170
20.2
8.4
55.3
1.5
4.1
186
21.9
8.5
55.1
1.1
Overall Means
157
19.9
55.0
3.4
5.7
174
7.8
54.1
1.9 CV
9.9
5.1
11.6
6.2
F-value
4.3
14.9
4.6
14.2
LSD (p=0.05)
16.8
20.9
1.4
Germplasm Enhancement of Maize
Suwan (Thai) x Cristalino Colorado (Argentina); top of test, low variance;

12. GEMN-0267 NEI9008:N0826(GEMN-0155)-B-095
Tester: HC33
Tester: MBS3520
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
NEI9008:N0826(GEMN-0155)-B-095 16
177
19.7
9.0
54.6
6.3
7.8 14
196
21.1
9.3
53.3
4.0
8.2
Test Entry Means
156
19.8
7.9
54.9
3.7
6.0
172
22.3
7.7
53.9
2.0
Check Means
170
20.2
8.4
55.3
1.5
4.1
186
21.9
8.5
55.1
1.1
Overall Means
157
19.9
55.0
3.4
5.7
174
54.1
1.9 CV
9.9
5.1
11.6
6.2
F-value
4.3
14.9
4.6
14.2
LSD (p=0.05)
16.8
20.9
1.4
Germplasm Enhancement of Maize
Suwan (Thai) x Cristalino Colorado (Argentina); 31D58 maturity; top of test, low variance ***

13. GEMS-0268 NS1:S0852-B-021 121307 Tester: MON-nSS
Tester: MBS8814
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
NS1:S0852-B-021 19
175
20.5
8.6
56.3
5.7
3.5 16
205
23.8
8.7
49.1
3.2
11.5
Test Entry Means
143
18.9
7.6
58.1
16.6
0.5
180
22.7
8.0
51.9
1.3
4.0
Check Means
154
20.9
7.4
58.2
0.2
186
21.4
54.1
1.7
2.7
Overall Means
181
22.6
52.1
3.9 CV
14.8
6.9
8.2
F-value
1.5
4.9
10.5
LSD (p=0.05)
29.7
2.0
14.9
LH287 tester in 2014 also; 199 bu, Y/M-8.1; Suwan (thailand); watch roots, but great yield and very large perse ears; moderate plant & ear ht; white cobs, good grain quality

14. GEMS-0269 NS1:S0852-B-054 Germplasm Enhancement of Maize
Tester: LH287
Tester: MBS8814
Pedigree
Ent
Yield B/A
% Moist
Y/M
TWT
% SL
% RL
NS1:S0852-B-054 21
187.6
22.2
8.6
52.5
2.1
9.65
197.2
21.9
9.0
51.7
0.9
9.5
Test Entry Means
186.2
23.3
8.0
53.4
1.9
7.8
180.5
22.7
51.9
1.3
4.0
Check Means
198.9
22.1
56.3
1.6
2.9
186.1
21.4
8.7
54.1
1.7
2.7
Overall Means
187.3
23.2
8.1
53.7
7.4
181.0
22.6
52.1
3.9 CV
9.2
4.3
8.2
5.7
F-value
2.8
15.3
3.2
10.5
LSD (p=0.05)
17.2
1.0
14.9
Germplasm Enhancement of Maize
Suwan (Thailand); high yielding, early line; high variance in hybrid combination

15. Top Cross Estimated Relative Maturity
Average
GEM Code
Pedigree
Estimated RM
Ear Height (cm)
Hybrid Checks
GEMN-0260
(GEMN-0140/GEMN-0097)-B-B-026
110
HC33xLH287
109
GEMN-0261
(GEMN-0140/GEMN-0097)-B-B-027 90
MBS3633xMBS8814
113
125
GEMS-0262
(GEMS-0147/GEMS-0180)-B
119
100
LH200xLH262
118
140
GEMS-0263
BR105:S1626(GEMS-0175)-B-049
116
34R65
~109
105
GEMS-0264
BR105:S
33F85
~114
GEMS-0265
DKXL212:S0950-B-083
31D58
~119
GEMN-0266
NEI9008:N0826(GEMN-0155)-B-074
130
GEMN-0267
NEI9008:N0826(GEMN-0155)-B-095
115
120
GEMS-0268
NS1:S0852-B-021
GEMS-0269
NS1:S0852-B-054 95

16. Above Average Resistance
GLS & Goss’s Wilt
Above Average Resistance
GLS
GEMS-0263
GEMS-0265
GEMS-0268
Goss’s Wilt
GEMS-0269
Germplasm Enhancement of Maize
2nd Goss’s Release
34 Lines All Years
Based on ratings from J. Dodd, PSR

17. Above Average Resistance
FUSER & Head Smut
Above Average Resistance
Germplasm Enhancement of Maize
FUSER
GEMS-0264
GEMN-0266
GEMN-0267
Head Smut
GEMS-0268
39 Lines All Years
47 Lines All Years
Based on ratings from M. Mancl, DuPont Pioneer (Woodland, CA)

18. Southern Leaf Blight Resistance (SLB)
Above Average Resistance
GEMS-0223
GEMN-0239
Germplasm Enhancement of Maize
87 Lines All Years
Based on ratings from P. Balint-Kurti, USDA-ARS PSRU

19. Questions?
Thanks to all Germplasm Enhancement Cooperators for their contributions and efforts. We sincerely hope that your role in the GEM Project has a positive impact on your program.
Please share your thoughts and ideas as to how GEM can better accomplish diversifying the genetic base of corn production and reduce crop vulnerability.

20. Thoughts as GEM transitions
New GEM Coordinator/Maize Geneticist needed in Ames
Experience with and passion for germplasm introgression
Superior communication and collaborative skills
Skill in use of statistical and IM tools relevant to plant breeding
Staff needs better analytical tools and to import data into database more readily.
Germplasm performance information needs to be publicly accessible via a database to supports user queries and information retrieval. Update as data becomes available.
GEM needs to leverage US and international network capacities to better evaluate and characterize germplasm in order to deploy appropriate genetic resources to address production challenges.

21. Thoughts continued…
*Cycle time must be reduced. This requires more resources , either internal or via collabors.
*Germplasm Access is becoming more challenging not less. Need to access unencumbered, valuable germplasm.
*Phytosanitary requirements for germplasm exchange are becoming more rigorous and more testing is required.
*AP testing and declarations may be required for exchange.
* Technical and managerial skills required for project execution are every bit as rigorous as for a commercial breeder but performed in an environment that is both federal and academic.

22. Best wishes to all in 2015!