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Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling  Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc.,

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Presentation on theme: "Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling  Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc.,"— Presentation transcript:

1 Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling  Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc., Woo Sik Lee, M.D., Ph.D., Kwang Ryul Cha, M.D., Tae Ki Yoon, M.D., Dong Ryul Lee, Ph.D.  Fertility and Sterility  Volume 91, Issue 5, Pages (May 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Cross-section of LN2 vapor tank. (A) LN2 vapor; (B) LN2.
Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Comparison of results obtained using LN2 and LN2 vapor storage in mouse embryos frozen by vitrification or slow cooling. (A) Survival rate after vitrification/warming. (B) Blastocyst formation rate after vitrification/warming. (C) Survival rate after slow-cooled/thawing. (D) Blastocyst formation rate after slow-cooled/thawing. (E) Percent of apoptotic cells in blastocysts after warming. (F) Total number of cells in blastocysts after warming. 1W = 1 week, 1M = 1 month, 6M = 6 months. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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