Presentation is loading. Please wait.

Presentation is loading. Please wait.

Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone  C. Pross, MD,

Similar presentations


Presentation on theme: "Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone  C. Pross, MD,"— Presentation transcript:

1 Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone  C. Pross, MD, M.M. Farooq, MD, J.S. Lane, MD, N. Angle, MD, C.K. Tomono, A.E. Xavier, MS, J.A. Freischlag, MD, A.E. Collins, PhD, R.E. Law, PhD, H.A. Gelabert, MD  Journal of Vascular Surgery  Volume 35, Issue 6, Pages (June 2002) DOI: /mva Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

2 Fig. 1 Smooth muscle cell migration. Dexamethasone inhibits rat aortic smooth muscle cell migration in dose-dependent fashion (n = 9). Dexamethasone does not alter human aortic smooth muscle cell migration (n = 9). *P <.05. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

3 Fig. 2 Apoptosis. Dexamethasone at 10−9 mol/L to 10−5 mol/L does not induce apoptosis of rat or human smooth muscle cells. Cell survival was uniform throughout all groups, as determined with flow cytometry (n = 3). Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

4 Fig. 3 MMP-2 secretion. Dexamethasone inhibits rat aortic smooth muscle cell MMP-2 secretion in dose-dependent manner (n = 3). Top, Western blot immunoassay. Densitometry (below). Dexamethasone does not effect human aortic smooth muscle cell MMP-2 secretion (n = 3). Bottom, Western blot immunoassay. Densitometry (below). *P <.05. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

5 Fig. 4 MMP-2 activity. Dexamethasone inhibits rat aortic smooth muscle cell MMP-2 activity in dose-dependent manner (n = 3). Top, Zymogram. Densitometry (below). Dexamethasone does not alter human aortic smooth muscle cell MMP-2 activity (n = 3). Bottom, Zymogram. Densitometry (below). *P <.05. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

6 Fig. 5 TIMP-2 secretion. Dexamethasone activates rat aortic smooth muscle cell TIMP-2 secretion in dose-dependent manner (n = 3). Top, Western blot immunoassay. Densitometry (below). Dexamethasone does not alter TIMP-2 secretion (n = 3). Bottom, Western blot immunoassay. Densitometry (below). *P <.05. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions


Download ppt "Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone  C. Pross, MD,"

Similar presentations


Ads by Google